Sequences from confirmation amplicon


Confirmation sequences for line 286A06 and hit At5g24520 are shown.
Line ID 286A06
Locus At5g24520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8371849
Primers used for PCR ZZ92 - 8474, show primer details
   
Sequence Name 01-K024728-022-286-A06C-8409
GenBank Accession LN486418 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K024728-022-286-A06C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGAATATATACTGTTGCTGGACCCAATGGGA
TTGATCCGATGTCGGTTTATTCGGCTGGTTCGGAGATTAATCAGTTGCAGTGGTCTTCTT
CGCAGCCTGATTGGATTGGTATTGCTTTTGCTAACAAAATGCAGCTCCTTAGAGTTTGAG
GTGAGAGTTTCTGTTTCGCTACATAATTCTCATTTGCTAGGCCTAGATTCTAATGAGGAA
GCATTGATTATTGGTTTAGATTGTGTTGCATTACAGATAGTCTTCTAGGTTTGGTAACTA
AACGTTTTTTCGATTCTTGATAACGAAGCCACTAGAGATTTGACACTAACTCGATTTAGA
TTTA
   
Sequence Name 02-K024728-022-286-A06C-ZZ92
GenBank Accession LN486874 [GenBank]
Sequencing primer gene specific primer, ZZ92: 5'-AAAAACCTGAAGCACACATACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K024728-022-286-A06C-zz92
GACCAGCATCAAGTTTCCAATTCCACTTCTATATGTTTCTGAGGGCATACAGCACAGTCA
GCAGTAAACAATTCTCAGATACTTCGTTAAATTAACAATATTTGTATACGTAGCTAGTGG
CTTCGTTAAATTAAGAATAGATTGTCTCAAAAAAAAATAGACTGAAGAATTATGGGTAAA
AATTAGAACCTGACGAGTGTACAACTATTCATCATATGATACATCAACTTAGATGAAAGC
CAACATATGACTACTACACTATCAGCCATTACAGCAAGTGTGAGACCTGTCGCTAATAAG
AGCCAATATATATGACTACTAAACTGTGATTTATGTATGCATAACAAAGTAATTGATTTT
AACAGAGATATTGATTCAGGTAAATCTAAAACGAGTTAGTGTCAAATCTCTAGTGGCTTT
GTTATCAAGAATCGAAAAAACGTTTAGTTACCAAACCTAGAAGACTATCTGTAATGCAAC
ACAATCTAAACCAATAATCAATGCTTCCTCATTAGAATCTAGGCCTAGCAAATGAGAATT
ATGTAGCGAAACAGAAACTCTCACCTCAAACTCTAAGGAGCTGCATTTTGTTAGCAAAAG
CAATACCAATCCAATCAGGCTGCGAAGAAGACCACTGCAACTGATTAATCTCCGAACCAG
CCGAATAAACCGACATCGGATCAATCCCATTGGGTCCAGCAACAGTATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAANAAGAAAGAGTAATTACCATTTTTTTTCATTCAAAA

Last Updated on 06.12.2018 16:11