Sequences from confirmation amplicon


Confirmation sequences for line 286E07 and hit At5g15700 are shown.
Line ID 286E07
Locus At5g15700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5121419
Primers used for PCR JS02 - 8474, show primer details
   
Sequence Name 20-K021167-022-286-E07C-8409
GenBank Accession LN495029 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K021167-022-286-E07C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCGTCTATTTTCTCGAAAAA
TTTGAGCTTTCGGTTTCCGGTCTTGGGATCACCATGTCACGGAAAGGGTTTTCGTTGTTT
AAGTGGGATCACGAGGCGAGAGGAGTTTTCCAAGAGCGAGAGGTGCCTTAGTGGGACTTT
GGCTAGAGGGTACACGAGTGTAGCCGAAGAGGAGGTCTTATCAACAGATGTTGAGGAAGA
GCCTGAGGTAGATGAATTGTTGAAGGAGATGAAGAAAGAGAAGAAGAGAGAAAGTCATCG
TTCGTGGCGGATGAAGAAGCAAGACCAATTTGGAATGGGTCGTACCAAGTTCCAGAATTT
ATGGAGAAGACAAGTTAAGATCGAGACTGAAGAATGGGAAAGAGCTGCTGCAGAGTACAT
GGAGCTTTTGACAGATATGTGTGAGCAAAAGCTTGCGCCTAATCTGCCTTATGTGAAGTC
TTTATTTCTGGGTTGGTTCGAACCATTAAGAGAT
   
Sequence Name 24-K021167-022-286-E07C-JS02
GenBank Accession LN496821 [GenBank]
Sequencing primer gene specific primer, JS02: 5'-TCAAGGTAATGTGCATAAGTTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K021167-022-286-E07C-JS02
CTTCCCTAATCTATACAACTCCTGGTCTTTAGCAATTGCATCTCTTAATGGTTCGAACCA
ACCCAGAAATAAAGACTTCACATAAGGCAGATTAGGCGCAAGCTTTTGCTCACACATATC
TGTCAAAAGCTCCATGTACTCTGCAGCAGCTCTTTCCCATTCTTCAGTCTCGATCTTAAC
TTGTCTTCTCCATAAATTCTGGAACTTGGTACGACCCATTCCAAATTGGTCTTGCTTCTT
CATCCGCCACGAACGATGACTTTCTCTCTTCTTCTCTTTCTTCATCTCCTTCAACAATTC
ATCTACCTCAGGCTCTTCCTCAACATCTGTTGATAAGACCTCCTCTTCGGCTACACTCGT
GTACCCTCTAGCCAAAGTCCCACTAAGGCACCTCTCGCTCTTGGAAAACTCCTCTCGCCT
CGTGATCCCACTTAAACAACGAAAACCCTTTCCGTGACATGGTGATCCCAAGACCGGAAA
CCGAAAGCTCAAATTTTTCCAGAA
Primers used for PCR FN49 - 8474, show primer details
   
Sequence Name 08-K035332-022-286-E07C-8409
GenBank Accession LN489640 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K035332-022-286-E07C-8409
GAGCCATTTAAATTGAATATTCCACGAGTTTGGGAACTGACGTTTAATCTAGCAGCTGAC
CTCGAAATTGCTTGTTTAGCAATGTTTCTCCACATGGGGAAGGATTGGGAAACTGGGTTT
GGAGAAGAAATAAAGGGTTTATGCAATGGAATCAAGTGATCGAATACCTTAGTCTGATTT
GCCAAGAACAGTGGGGTTTGAGCACTGGACATCAATCGAACTTAAAAATTGAGTAAAATC
GATAAACCAGAACAATAACCAACTCGAGATTGAATAAATTCGATCGCATGAGCAAAGAAA
AATGGCAAATTGGGAAGAGTTTCATAGACGGAAGAAAATAAAAACAAATGGTGAGTGAAG
CGTACACGACGAAGCCGCCTTAAACCCTCGCTTAAAAACCCTTGCTTTCTTAAGCCTCTG
TTTTTTTTTTCTGGGTTGTCTCTGTTTCTCACATGTCAAACAATTAAAAAATAAATTAAA
AATATCTACCCTAAATTCGACCCGTAAAGGAGGATCTTACCCCACCCGGGAAGTATCTAA
TTAAGGCC
   
Sequence Name 08-K035332-022-286-E07C-FN49
GenBank Accession LN489641 [GenBank]
Sequencing primer gene specific primer, FN49: 5'-TAGTAAAGAGACTTTGGGCCGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K035332-022-286-E07C-FN49
GGGTCGGGTAGATCCTCCTATACGGGTCGAATTTAGGGTAGATATTTTTAATTTATTTTT
TAATTGTTTGACATGTGAGAAACAGAGACAACCCAGAAAAAAAAAACAGAGGCTTAAGAA
AGCAAGGGTTTTTAAGCGAGGGTTTAAGGCGGCTTCGTCGTGTACGCTTCACTCACCATT
TGTTTTTATTTTCTTCCGTCTATGAAACTCTTCCCAATTTGCCATTTTTCTTTGCTCATG
CGATCGAATTTATTCAATCTCGAGTTGGTTATTGTTCTGGTTTATCGATTTTACTCAATT
TTTAAGTTCGATTGATGTCCAGTGCTCAAACCCCACTGTTCTTGGCAAATCAGACTAAGG
TATTCGATCACTTGATTCCATTGCATAAACCCTTTATTTCTTCTCCAAACCCAGTTTCCC
AATCCTTCCCCATGTGGAGAAACATTGCTAAACAAGCAATTTCGAGGTCAGCTGCTAAAT
AAAACGTCAGTTCCCAAACTCGTGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTA

Last Updated on 06.12.2018 16:11