Sequences from confirmation amplicon


Confirmation sequences for line 286G11 and hit At1g72570 are shown.
Line ID 286G11
Locus At1g72570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27332594
Primers used for PCR PH34 - 8474, show primer details
   
Sequence Name 09-K017512-022-286-G11C-PH34
GenBank Accession LN489965 [GenBank]
Sequencing primer gene specific primer, PH34: 5'-TGTTATTGAGTTCCTCGATCTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K017512-022-286-G11C-PH34
CTCAACTGAATAAAAGAGCGTCAAAGGAACTGATATCACAAGCCAAATCTATGTAGTTCC
ATCAAAATCAAGTCAATAAAATCGGAAAATGATTACATTCAAGATCCAAAATATCGAAAT
AAGTATGGAAACTGTTATGTTGCATGTATATAAGTGTACATATATAAAACACACTTGCAT
GTAATAAAGGAGTTAGAAGTGGAGCTAGGTACGGGACCAGAAAGATCAAACTCACAGGGA
AATTTAAGTGAGTGGTAGGACCCCAATACTTCAGAGCCGCTAAATCATATGCCCTCGCTG
CTTTCTCCTCCTCATCATACCCTCCTATTTTAGTAAGTATTAATTCTACTATTAAATGTT
TTTCTATAATTATCAGAAGGTAACAAGGTTAATTTTTACTTCGAAGGCCAAAAATAGGGC
AACAAGAAATGAACGAGGGATCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 13-K017512-022-286-G11C-8409
GenBank Accession LN491825 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K017512-022-286-G11C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAGATCCCTCGTTCATT
TCTTGTTGCCCTATTTTTGGCCTTCGAAGTAAAAATTAACCTTGTTACCTTCTGATAATT
ATAGAAAAACATTTAATAGTAGAATTAATACTTACTAAAATAGGAGGGTATGATGAGGAG
GAGAAAGCAGCGAGGGCATATGATTTAGCGGCTCTGAAGTATTGGGGTCCTACCACTCAC
TTAAATTTCCCTGTGAGTTTGATCTTTCTGGTCCCGTACC

Last Updated on 06.12.2018 16:11