Sequences from confirmation amplicon


Confirmation sequences for line 287E03 and hit At5g62440 are shown.
Line ID 287E03
Locus At5g62440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25074088
Primers used for PCR MD02 - 8474, show primer details
   
Sequence Name 11-K022912-022-287-E03C-8409
GenBank Accession LN490927 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K022912-022-287-E03C-8409
GTAGATTTCCCGGACATGAAGCCAAAAAATCAAATAGACCGAACCAGTTCGGTTCAATCG
GAAACCAAAGTCATATAAATATACCGCTTAGACGATTTCTGTAGGTCACACACCCTCAAG
CTTAAACCCTAGAAATCGTAGAAAGGTGAAAAAAAGGCGGAAGCTTTACTAGCTTAACAA
TGGCTGAAGAACAAGAGATCGTCGATTCCCTGTCAGCGGAAGTGAACCCAGACCAGAAAG
TGGACATGGAAGTCGAAACCGCGACCCCGAAAGCCGAGACCGGCGATGAGAAACGTGAGA
GAGAGGAGACAGAGGAAGAAGAGAACGGAGGTGAATCGAAGAAGCAGAAGGTTGGAGAGG
AAGAGAAGTCAGGTCCGGTTAAACTTGGTCCCAAGGAGTTTGTTACCTCTGTGGCGATGT
TCGATTACTTTGTCAAGTTTTTACACTTTTGGCCAACTGATCTCGATGTTAATAAGGTAA
TACATATTTGGGTTTACT
   
Sequence Name 15-K022912-022-287-E03C-MD02
GenBank Accession LN492782 [GenBank]
Sequencing primer gene specific primer, MD02: 5'-CAAAGACGAAGAGTAAACCCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K022912-022-287-E03C-MD02
ATGTATTAACCTTATTAACATCGAGATCAGTTGGCCAAAAGTGTAAAAACTTGACAAAGT
AATCGAACATCGCCACAGAGGTAACAAACTCCTTGGGACCAAGTTTAACCGGACCTGACT
TCTCTTCCTCTCCAACCTTCTGCTTCTTCGATTCACCTCCGTTCTCTTCTTCCTCTGTCT
CCTCTCTCTCACGTTTCTCATCGCCGGTCTCGGCTTTCGGGGTCGCGGTTTCGACTTCCA
TGTCCACTTTCTGGTCTGGGTTCACTTCCGCTGACAGGGAATCGACGATCTCTTGTTCTT
CAGCCATTGTTAAGCTAGTAAAGCTTCCGCCTTTTTTTCACCTTTCTACGATTTCTAGGG
TTTAAGCTTGAGGGTGTGTGACCTACAGAAATCGTCTAAGCGGTATATTTATATGACTTT
GGTTTCCGATTGAACCGAACTGGTTCGGTCTATTTGATTTTTTGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACG
Primers used for PCR MD03 - 8474, show primer details
   
Sequence Name 35-K022913-022-287-E03C-8409
GenBank Accession LN501664 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K022913-022-287-E03C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCACTACATGAAAACCAGGA
TTTTCAATGTAGTAACTAATTTGTCGTCATTGATCTGTCCAATTATTATTTTTACGTCTC
TTAAATCTTTTCATTTTAAAGTCTACATTTTTCTAATATATTTTTTTATTAATCATCTCA
AGATATTTTTTTAGTCTACAAATTACTTTTACTGTTGTAGAAAATTTGGATAATAGTCAG
TAATTCTAAATAATAGAATAAAAACTCAAATCTTAATATAATAGTTTAACAAATAAATCA
TATTATACTTTTTTTGGATAAAAAATGTTGATATAATTTACAATGTTATTAAAAATAATT
TTTGGGATACCTAAACGTTTTAGATGTTCAGTCTGTCCGGGAT
   
Sequence Name 39-K022913-022-287-E03C-MD03
GenBank Accession LN503210 [GenBank]
Sequencing primer gene specific primer, MD03: 5'-TATCCCGACAGACTGAACATCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K022913-022-287-E03C-MD03
GTTTAGGTATCCCAAAAATTATTTTTAATAACATTGTAAATTATATCAACATTTTTTATC
CAAAAAAAGTATAATATGATTTATTTGTTAAACTATTATATTAAGATTTGAGTTTTTATT
CTATTATTTAGAATTACTGACTATTATCCAAATTTTCTACAACAGTAAAAGTAATTTGTA
GACTAAAAAAATATCTTGAGATGATTAATAAAAAAATATATTAGAAAAATGTAGACTTTA
AAATGAAAAGATTTAAGAGACGTAAAAATAATAATTGGACAGATCAATGACGACAAATTA
GTTACTACATTGAAAATCCTGGTTTTCATGTAGTGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11