Sequences from confirmation amplicon


Confirmation sequences for line 288G04 and hit At2g23480 are shown.
Line ID 288G04
Locus At2g23480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10003020
Primers used for PCR FV88 - 8474, show primer details
   
Sequence Name 09-K100894-0022-288-G04C-8409
GenBank Accession LN490100 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K100894-0022-288-G04C-8409
ATCTGTGTATACTGGTAATTACGGGGAATACTTTTAGGAAATACAAAGTAATAATGTGGC
AGTACTATTTACAATTGAGTGATATATGAATAGGAGTATCTGTGTAATACTGGTAATTAC
GGGGAATACTTTTTGGAAATACAGAGTAATAATGTGGCAGTACTATTTACAATTGAGTGA
TATATAAATGAATGTTTGTTTCTTTCAGGATATGGAGGAAGCGATGGCAATGCAGCCACT
TGGTATGTACTTCCCGGCGTCGGAGTATACCAAGAAGATGAAGTTAGCAACGAGGTGTTA
TATCTCGGAGGTGTTGAAGACCTTCGCTGACTTGGAACATCCTCTAACGAATGTTGAAAA
GAATTATTTTATGGAGCATCCGAGTTTCAAGCACATCTACCACCTGCCTTCCGGATATAC
CCACAAGTTAATGGGGATGTGGATGCTTTTTCTTCGAACGGCATCTATTGAGAAGAAAAA
GGAGGTTTGGTT
   
Sequence Name 09-K100902-0022-288-G04C-FV88
GenBank Accession LN490101 [GenBank]
Sequencing primer gene specific primer, FV88: 5'-AACTCCATTAACAACGAACCAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K100902-0022-288-G04C-FV88
TGCCGTTCGAGAAAAGCATCCACATCCCCATTAACTTGTGGGTATATCCGGAAGGCAGGT
GGTAGATGTGCTTGAAACTCGGATGCTCCATAAAATAATTCTTTTCAACATTCGTTAGAG
GATGTTCCAAGTCAGCGAAGGTCTTCAACACCTCCGAGATATAACACCTCGTTGCTAACT
TCATCTTCTTGGTATACTCCGACGCCGGGAAGTACATACCAAGTGGCTGCATTGCCATCG
CTTCCTCCATATCCTGAAAGAAACAAACATTCATTTATATATCACTCAATTGTAAATAGT
ACTGCCACATTATTACTCTGTATTTCCAAAAAGTATTCCCCGTAATTACCAGTATTACAC
AGATACTCCCATTCATATATCACTCAATTGTAAATAGTACTGCCACATTATTACTTTGTA
TTTCCTAAAAGTATTCCCCGTAATTACCAGTATTACACAGATACTCCTATTTTACTATAT
ACCTGGATTAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTA

Last Updated on 06.12.2018 16:11