Sequences from confirmation amplicon


Confirmation sequences for line 294B06 and hit At2g34470 are shown.
Line ID 294B06
Locus At2g34470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14531591
Primers used for PCR HM03 - 8474, show primer details
   
Sequence Name 11-K028846-022-294-B06C-8409
GenBank Accession LN490976 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K028846-022-294-B06C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAAGATATCCGTATCAATTTGGGTCCT
CTTGAGGAGTTATCTAACTTATTCAAGGCTGATTTGCTTCTTTGTGAATCCGGTGGAGGT
GAGGTTTCATTTCTAGCTAGTTCTTGATAGTTAATCTGCACGATATATAGATTTCTGTTT
CTCAAGGAACCAATGAGATATGAATAGGCTTAGTGTTGTGTGTTGCAGATAATTTAGCTG
CTAATTTCAGTAGAGAACTAGCTGACTATATCATCTACATCATCGATGTATCGGCTGGTG
ATAAAATACCCCGTAAAGGCGGCCCTGGAATCACTCAAGCTGATCTTCTGGTGCGCTTAT
GCTGCCTTAACTCTTCACTTACTCCTTTTGATCAAGTCTATAGTAGTGTGAAAGAATCTC
TTGTGGATTTTTCCAACTGCAGGTGATAAACAAGACTGAACTTG
   
Sequence Name 12-K028846-022-294-B06C-HM03
GenBank Accession LN491436 [GenBank]
Sequencing primer gene specific primer, HM03: 5'-TGCAAGGTCAGTCTTGTTTATCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K028846-022-294-B06C-HM03
CAGTTGGAAAAATCCACAAGAGATTCTTTCACACTACTATAGACTTGATCAAAAGGAGTA
AGTGAAGAGTTAAGGCAGCATAAGCGCACCAGAAGATCAGCTTGAGTGATTCCAGGGCCG
CCTTTACGGGGTATTTTATCACCAGCCGATACATCGATGATGTAGATGATATAGTCAGCT
AGTTCTCTACTGAAATTAGCAGCTAAATTATCTGCAACACACAACACTAAGCCTATTCAT
ATCTCATTGGTTCCTTGAGAAACAGAAATCTATATATCGTGCAGATTAACTATCAAGAAC
TAGCTAGAAATGAAACCTCACCTCCACCGGATTCACAAAGAAGCAAATCAGCCTTGAATA
AGTTAGATAACTCCTCAAGAGGACCCAAATTGATACGGATATCTTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTTCCCATTTTTTTTCCATTCCAA

Last Updated on 06.12.2018 16:11