Sequences from confirmation amplicon


Confirmation sequences for line 295B12 and hit At3g45443 are shown.
Line ID 295B12
Locus At3g45443 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16669668
Primers used for PCR 81wk - 3144, show primer details
   
Sequence Name 33-K118366-0022-295-B12C-81wk
GenBank Accession LN501182 [GenBank]
Sequencing primer gene specific primer, 81wk: 5'-CAGATTGCTTTGTTGTATGTTCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K118366-0022-295-B12C-81wk
ATTGAGTTGTTCTGTGAAGCAGTAGCAAAGACGGAGGGTGATGATACGGAGATAGAGGAG
CTTGTCAAAGACTTCCCTGATATTTTTGCTGAACCAACTGAATTGCCTCCATTTCGGACT
CAACATGACCACAAAATTGAGTTGTTGGAGGGCTCTAATCCCGTTAATCAACGACCCTAT
CGGTACTCTATTCACCAGAAGAATGAGATTGACAAGTTGGTTGAAGAGATGTTACAAAGT
GGTACAGTTCAAGTAAGTTCTAGTCCTTATGCTTCACCGGTGGTGCTAGTCAAAAAGAAG
GATGGATCTTGGCGTTTATGTGTTGATTATCGGGAACTCAATGGAATGACTGTGAAAGAT
AGGTTTCCAATTCCTCTTATTGAAGATCTAATGGATGAACTGGGAGGGTCTGTTGTCTTC
TCTAAGATTGATTTACGTGAAGGATATCATCAAGTGCGATTGAAAAATAATAGTAGAAGG
CAGTGAATTGTTTTACGAATGTGAAAGACCAAAATTTTAGACTTAAGCATACATGGCAAA
AGGAGAGACTTGCAGTTTTGGGAAAAGTGACTGTTGATTTAGTATAATTAGATGAAAAGT
AAACTGGTAGATTAATTAAGGTAATAATAAAATAACACGAGAGTTGAACCAAAAAACAAA
TCAATTGTAAACCGAAGCCATTTACAATTGAATATAAACACTGATAGTTTAAACCGAAGG
CGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTT
ATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGG
AGATAT
   
Sequence Name 54-K118365-0022-295-B12C-35St
GenBank Accession LN508625 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K118365-0022-295-B12C-35St
TTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGACGATTGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTATATTCAATTGTAA
ATGGCTTCGGTTTACAATTGATTTGTTTTTTGGTTCAACTCTCGTGTTATTTTATTATTA
CCTTAATTAATCTACCAGTTTACTTTTCATCTAATTATACTAAATCAACAGTCACTTTTC
CCAAAACTGCAAGTCTCTCCTTTTGCCATGTATGCTTAAGTCTAAAATTTTGGTCTTTCA
CATTCGTAAAACAATTCACTGCCTTCTACTATTATTTTTCAATCGCACTTGATGATATCC
TTCACGTAAATCAATCTTAGAGAAGACAACAGACCCTCCCAGTTCATCCATTAGATCTTC
AATAAGAGGAATTGGAAACCTATCTTTCACAGTCATTCCATTGAGTTCCCGATAATCAAC
ACATAAACGCCAAGATCCATCCTTCTTTTTGACTAGCACCACCGGTGAAGCATAAGGACT
AGAACTTACTTGAACTGTACCACTTTGTAACATCTCTTCAACCAACTTGTCAATCTCATT
CTTCTGGTGAATAGAGTACCGATAGGGTCGTTGATTAACGGGATTAGAGCCCTCCAACAA
CTCAATTTTGTGGTCATGTTGAGTCCGAAATGGAGGCAATTCAGTTGGTTCAGCAAAAAT
ATCAGGGAAGTCTTTGACAAGCTCCTCTATCTCCGTATCATCACCCTCCGTCTTTGCTAC
TGCATTCACAGAACAACTCAATCCCTGTTCCAACAACTCTTCTTCTGCTGGCACTTCACG
AACATACAC

Last Updated on 06.12.2018 16:11