Sequences from confirmation amplicon


Confirmation sequences for line 296E07 and hit At4g02890 are shown.
Line ID 296E07
Locus At4g02890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1279761
Primers used for PCR PF14 - 8474, show primer details
   
Sequence Name 38-K102871-0022-296-E07C-PF14
GenBank Accession LN502937 [GenBank]
Sequencing primer gene specific primer, PF14: 5'-AAAAGTTACGTGTCAAGCTTTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K102871-0022-296-E07C-PF14
GACTCGTCCTCGTCTTTTTTTCTTCTTCTATAAATTAATAACCTAAGACCTATTCTTCTT
CACAAATTCAGATTCCAAATTTCTCAAACTCTGAAATCGTTCTTTCAAATCTCTCAATCG
GGATCAAGGTAAATTTCTGGGTTCTTATTCAGTTTCTTCGATTATTTTCCATTCGATCGC
AATTTCGTCCCTGTTCTTTGAGTTTGATTCGGTTAATCTTTGATCGGAGATAAATATCTG
AGTTTCATCGCTAGATCTCATCTATATTCTGAACTAGGGTTCGTAGATTTCACAGATTTG
TATATTTAGGCGTCGATTCTTTGAATTTGCCTCCTTCGCAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTACACATTCACG
TCCAAATGGGAA
   
Sequence Name 38-K102876-0022-296-E07C-8409
GenBank Accession LN502938 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K102876-0022-296-E07C-8409
TTCGGACTGAGCCTTTACATTGAAATATCCTGCGAAGGAGGCAAATTCAAAGAATCGACG
CCTAAATATACAAATCTGTGAAATCTACGAACCCTAGTTCAGAATATAGATGAGATCTAG
CGATGAAACTCAGATATTTATCTCCGATCAAAGATTAACCGAATCAAACTCAAAGAACAG
GGACGAAATTGCGATCGAATGGAAAATAATCGAAGAAACTGAATAAGAACCCAGAAATTT
ACCTTGATCCCGATTGAGAGATTTGAAAGAACGATTTCAGAGTTTGAGAAATTTGGAATC
TGAATTTGTGAAGAAGAATAGGTCTTAGGTTATTAATTTATAGAAGAAGAAAAAAAGACG
AGGACGAGTCGGTCACGAGAAAGCTAAGGCGGTGAAGCAATAACCAACCAAAGCTTGACC
GNTAACTT

Last Updated on 06.12.2018 16:11