Sequences from confirmation amplicon


Confirmation sequences for line 296F08 and hit At3g14210 are shown.
Line ID 296F08
Locus At3g14210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4731730
Primers used for PCR JA37 - 8474, show primer details
   
Sequence Name 04-K100994-0022-296-F08C-8409
GenBank Accession LN487843 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K100994-0022-296-F08C-8409
GAGCCTTTACATTGAATATATCCTGTCCAGTGATTACCAAAATTAGAGGATACAACAGAT
ATTCAAAAGCAGTTTATTCGACAACGTCAAAGGCATAATTTGACGTCATATAGGCATAAT
TCCACGTTACGATATCGATTATACATTATAAGACAATAGCTCTCATATATATCGAAATAT
TCAGCTCAAAGCCATCCAACTAATAATAGGCGGCGAGGCCCAAGTAAAAGTCACGAGACG
GCCGACGACGGATAGCCAAGTTGTTGGGTTCCCAATACTCACGCAAGATTTCTCC
   
Sequence Name 04-K100998-0022-296-F08C-JA37
GenBank Accession LN487844 [GenBank]
Sequencing primer gene specific primer, JA37: 5'-TGGAGAAACTATGCGTGAGTATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K100998-0022-296-F08C-JA37
GCCGTCTCGTGACTTTTACTTGGGCCTCGCCGCCTATTATTAGTTGGATGGCTTTGAGCT
GAATATTTCGATATATATGAGAGCTATTGTCTTATAATGTATAATCGATATCGTAACGTG
GAATTATGCCTATATGACGTCAAATTATGCCTTTGACGTTGTCGAATAAACTGCTTTTGA
ATATCTGTTGTATCCTCTAATTTTGGTAATCACTTGGACAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11