Sequences from confirmation amplicon


Confirmation sequences for line 297D04 and hit At1g51190 are shown.
Line ID 297D04
Locus At1g51190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18979579
Primers used for PCR 05hd - 8474, show primer details
   
Sequence Name 41-K112477-0022-297-D04C-05hd
GenBank Accession LN504131 [GenBank]
Sequencing primer gene specific primer, 05hd: 5'-TAAAGGCTGATGATGATGAAGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K112477-0022-297-D04C-05hd
GTAGGTTGAGCTGAACCTAGAGCTGGAAGCACAGAGCTCGAGCCGCTCGCTGCACCATAT
TGATGGAAATTTGATCCGAGGGCTATCATTTCCTCTCGTTTTCTTGATGATTCTAGAGCT
TGAGCTTCTTTGAGCCGTTTAGCCGCACCACCTCCTATAGGAAGTGTGTTGCTCTCCAGG
ATGGCTTTCACATCATACCGATTTATCTCAAAGTTTGTAACCGCGTTTAGACCTCGAAAC
TTTATCGCAGCTATGTCATAAGCTTCTGCTGCTTCTGCCGCGTTTGAATATATATCGCAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCACGATTATTA
   
Sequence Name 18-K112476-0022-297-D04C-8409
GenBank Accession LN494370 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K112476-0022-297-D04C-8409
TCGGATGAGCATTTCATTGAATTATCCTGCGATATATATTCAAACGCGGCAGAAGCAGCA
GAAGCTTATGACATAGCTGCGATAAAGTTTCGAGGTCTAAACGCGGTTACAAACTTTGAG
ATAAATCGGTATGATGTGAAAGCCATCCTGGAGAGCAACACACTTCCTATAGGAGGTGGT
GCGGCTAAACGGCTCAAAGAAGCTCAAGCTCTAGAATCATCAAGAAAACGAGAGGAAATG
ATAGCCCTCGGATCAAATTTCCATCAATATGGTGCAGCGAGCGGCTCGAGCTCTGTTGCT
TCCAGCTCTAGGCTTCAGCTTCAACCTTACCCTCTAAGCATTCAACAACCTTTTGAGCAT
CTTCATCATCATCAGCCTTT

Last Updated on 06.12.2018 16:11