Sequences from confirmation amplicon


Confirmation sequences for line 298A05 and hit At1g75850 are shown.
Line ID 298A05
Locus At1g75850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28478477
Primers used for PCR CD26 - 8474, show primer details
   
Sequence Name 07-K104193-0022-298-A05C-CD26
GenBank Accession LN489250 [GenBank]
Sequencing primer gene specific primer, CD26: 5'-CTTGTAAAGAAGGGATCTGAGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K104193-0022-298-A05C-CD26
TTGTTGATCAATTCTATGAGGCTCTGTATATCAGATGGAGTGATATGTGGATTCCCTTTC
TCAAAGAAGTAAATGTACCTGTTGTATATGAAAAAAACATTTTTAGTATTGAGATGCTGC
TTTGTGTATGAATCTGTGTGTCAGATGTTTTTGTTTGTTTGTTGTTAAGTATACTGACTT
GTTCAAGATTTCAACAAACAGCGTGACTGGTCCGCTGCTACCTCTGGTTGCACTAGCCAT
TTGTTGAGCTGCGTTCGCTATCCTCAGTGCACGTCTTAAACAGAGAAGAACTACAAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 23-K104194-0022-298-A05C-8409
GenBank Accession LN496534 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K104194-0022-298-A05C-8409
CCATTTTGTAGTTCTTCTCTGTTAAGACGTGCACTGAGGATAGCGAACGCAGCTCAACAA
ATGGCTAGTGCAACCAGAGGTAGCAGCGGACCAGTCACGCTGTTTGTTGAAATCTTGAAC
AAGTCAGTATACTTAACAACAAACAAACAAAAACATCTGACACACAGATTCATACACAAA
GCAGCATCTCAATACTAAAAATGTTTTTTTCATATACAACAGGTACATTTACTTCTTTGA
GAAAGGGAATCCACATATCACTCCATCTGATATACAGAGCCTCATAGAATTGATCAACAA
TGAGATGCAAAGCGACAACGGTAACACGACAATCCACTCAGATCACTTNTTTACAA

Last Updated on 06.12.2018 16:11