Sequences from confirmation amplicon


Confirmation sequences for line 302F10 and hit At5g20040 are shown.
Line ID 302F10
Locus At5g20040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6768525
Primers used for PCR MI07 - 8474, show primer details
   
Sequence Name 37-K018492-022-302-F10C-MI07
GenBank Accession LN502442 [GenBank]
Sequencing primer gene specific primer, MI07: 5'-GCATGTCCAGTTCAGTGAAGAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K018492-022-302-F10C-MI07
GGATTTGGTTCTAGAAATTGCATGACTCCATGTTATATAACAGTTATTTTTCGGTTTAAG
ACATATAGCCATTACTATTAGCATATATCATCATCCACTTATACCAAGTTGGTTCAGAAA
GGAAGTCCATTCTTATTTAGTTGGAAAAATGAAATACTTTACGACTTCATCATTGCAATT
TATTTGTCGTACTTTGATACCGTTTTTGATCAACAATGGAAATATGATTCCTTGTTTTCA
GGCCATGGAATATCTATTACAATGTCGTCGATATGAGGGCGAAAGTTCCCCTAGAGAATT
TTATGCTTTCTTGAACAAATTTCAGACAGCATCCAGGTGAATCCTCTACATAGACTTGAA
ATATCTAGTGTATTAGCTTTTGGACGAAATAGTGATTGAGAATAAATGAAAGTCGAAGTT
CTGTTTTGATTCTTTCACATTGATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
   
Sequence Name 38-K018492-022-302-F10C-8409
GenBank Accession LN502820 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K018492-022-302-F10C-8409
TCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATCAATGTGAAAGAATCAAA
ACAGAACTTCGACTTTCATTTATTCTCAATCACTATTTCGTCCAAAAGCTAATACACTAG
ATATTTCAAGTCTATGTAGAGGATTCACCTGGATGCTGTCTGAAATTTGTTCAAGAAAGC
ATAAAATTCTCTAGGGGAACTTTCGCCCTCATATCGACGACATTGTAATAGATATTCCAT
GGCCTGAAAACAAGGAATCATATTTCCATTGTTGATCAAAAACGGTATCAAAGTACGACA
AATAAATTGCAATGATGAAGTCGTAAAGTATTTCATTTTTCCAACTAAATAAGAATGGAC
TTCCTTTCTGAACCAACTTGGTATAAGTGGATGATGATATATGCTAATAGTAATGGCTAT
ATGTCTTAAACCGAAAAATAACTGTTATATAACATGGAGTCATGCAATTTCTAGAACCAA
ATCCATATTCTTCACTGAATGGGACAT

Last Updated on 06.12.2018 16:11