Sequences from confirmation amplicon


Confirmation sequences for line 302G08 and hit At2g33610 are shown.
Line ID 302G08
Locus At2g33610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14229073
Primers used for PCR CK28 - 8474, show primer details
   
Sequence Name 43-K017886-022-302-G08C-CK28
GenBank Accession LN504674 [GenBank]
Sequencing primer gene specific primer, CK28: 5'-GTCAGTGAAACTAATCTTCCGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K017886-022-302-G08C-CK28
AGGTCGTCTCTGTACTGCTTAATAATCGAGTTCCTCAAGTAGAGATAAAACTTAGGGTTT
TTGGATGAAGATCTCGAATCGAAAAATTCCGGCAGTGACCGGACCTCGCAATCGTTAATG
TCGGTCCATGAAAACCAACCTAATTGGTTAAATAGACGGTACAAGTACAAAATCCGTAAG
AAACAGTGAAGAAAGTAGAATAAATCAAAGTTTTCTCGGCGTTGCAGACTTACTGGAGTA
GCTAGGTACGTGAATATTATCGATATCGGAAGAAGAAGACGGCAATTGAGCCGACTTAGA
AGCTGCTGCGGCGCCTCCAGACGTAGTTTCGGAAAGAGAGGGAGTGTCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
ACATTC
   
Sequence Name 44-K017886-022-302-G08C-8409
GenBank Accession LN505030 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K017886-022-302-G08C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGACACTCCCTCTC
TTTCCGAAACTACGTCTGGAGGCGCCGCAGCAGCTTCTAAGTCGGCTCAATTGCCGTCTT
CTTCTTCCGATATCGATAATATTCACGTACCTAGCTACTCCAGTAAGTCTGCAACGCCGA
GAAAACTTTGATTTATTCTACTTTCTTCACTGTTTCTTACGGATTTTGTACTTGTACCGT
CTATTTAACCAATTAGGTTGGTTTTCATGGACCGACATTAACGATTGCGAGGTCCGGTCA
CTGCCGGAATTTTTCGATTCGAGATCTTCATCCAAAAACCCTAAGTTTTATCTCTACTTG
AGGAACTCGATTATTAAGCAGTACAGAGACGACCATCCTCGGAAGATTAG

Last Updated on 06.12.2018 16:11