Sequences from confirmation amplicon


Confirmation sequences for line 302G09 and hit At1g76420 are shown.
Line ID 302G09
Locus At1g76420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28673480
Primers used for PCR CV01 - 8474, show primer details
   
Sequence Name 26-K021167-022-302-G09C-8409
GenBank Accession LN497727 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K021167-022-302-G09C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATTAGAGAGAGTGGTACTT
TTATAGTCTAAGGGACAGGAAATATCCGACAGGTTTGAGGACTAACAGAGCAACTACTGC
TGGATACTGGAAAGCTACCGGCAAAGATAAGGAAGTCTTCTCCGGCGGAGGAGGACAGCT
TGTTGGGATGAAGAAGACGTTGGTGTTCTACAAAGGTAGGGCTCCACGTGGCCTCAAGAC
TAAGTGGGTCATGCATGAGTATCGCCTCGAAAACGACCATTCACACCGCCACACGTGTAA
GGTAAATTAACACTCTTTTACTCTCACTCGTGCGTCTCACTCTCACTCGTGCATCTCTTT
CTTTAAAAAAATTTGTATTTTTTCTCTTGTCAATTTATGAGTTGACTTTTTGAAACCAAA
AATTTTATACTACTAGTGTGTGAAACTAGTAATTTAAACCCTGTTACTTTTATTCCCACA
CGTCCATTTCTTATAACATTAAATTCTTTTTAACTTTGCGAATGTAATAGAAATTTGATG
TTTTTGTTCTCCATTAATGTGTTAACTTTTTAAATCCACAAGTTGAAAAGAAAGGTTTAA
CGAAATATTTAACTTTTTTTTTCCCATTTCA
   
Sequence Name 30-K021167-022-302-G09C-CV01
GenBank Accession LN499541 [GenBank]
Sequencing primer gene specific primer, CV01: 5'-ATTGAAACAAAACACATGAAATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K021167-022-302-G09C-CV01
AAAAAAGTTAAATATTTCGTTAAACCTTTCTTTTCAACTTGTGGATTTAAAAAGTTAACA
CATTAATGGAGAACAAAAACATCAAATTTCTATTACATTCGCAAAGTTAAAAAGAATTTA
ATGTTATAAGAAATGGACGTGTGGGAATAAAAGTAACAGGGTTTAAATTACTAGTTTCAC
ACACTAGTAGTATAAAATTTTTGGTTTCAAAAAGTCAACTCATAAATTGACAAGAGAAAA
AATACAAATTTTTTTAAAGAAAGAGATGCACGAGTGAGAGTGAGACGCACGAGTGAGAGT
AAAAGAGTGTTAATTTACCTTACACGTGTGGCGGTGTGAATGGTCGTTTTCAAGGCGATA
CTCATGCATGACCCACTTAGTCTTGAGGCCACGTGGAGCCCTACCTTTGTAGAACACCAA
CGTCTTCTTCATCCCAACAAGCTGTCCTCCTCCGCCGGAGAAGACTTCCTTATCTTTGCC
GGTAGCTTTCCAGTATCCAGCAGTAGTTGCTCTGTTAGTCCTCAAACCTGTCGGATATTT
CCTGTCCCTTAGACTATAAAAGGACCACTTCTCTCTAATTCAATTGAAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAA

Last Updated on 06.12.2018 16:11