Sequences from confirmation amplicon


Confirmation sequences for line 305A06 and hit At2g22630 are shown.
Line ID 305A06
Locus At2g22630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9620680
Primers used for PCR PH40 - 8474, show primer details
   
Sequence Name 45-K017546-022-305-A06C-PH40
GenBank Accession LN505365 [GenBank]
Sequencing primer gene specific primer, PH40: 5'-TACTTTTCTCGACAATTCGAGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K017546-022-305-A06C-PH40
ATGATGAACAAGATTCCTCTGCAAAAGATAAAACACTTATAAGAACCTGAAAACTAGAAA
TGATAACATTGATTAATAATGCGTTTCAAACTTAAACACAACATCTTTAGTATCTTTTCT
CAAAGAACAGATTTTTTTGTAAATTGCAAATGCAAACACAAACCGAAGAAGAAACTAAAA
ATATCGTTTGCATTTTACATCTGCAAATTATGTGTGTTTACAATGTAAACACAAACAACA
ACATCCGAGTCTGATTTTTAATAATCATGTGTTATATCATATAGTTTACCTTTCTGGTTA
GCTCTTTAATTTCATTGGTCAAAATTTGTTCCTGCAAGGTTCGAAAAGAATAATTAGCTT
AAACTTCAATGCTCTATTATCAATTTTTAAGCATCATGCGAACCAGCTAGAGGCCTAGAG
CATTCATATATTACCCTTTTCATACGAATTCCACGTAAACTCATTTCAAGTTGACTCTCT
ATGTTTTGTAACTCCTTAACGCTCAAACCATTTAATTCCACTCCCGTTAGTTGCCTGATT
TATCACAAAACATTACTGCAGTTAATATATGTAGAACGGACATGGCCAACTACAACCAAT
TTAAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
   
Sequence Name 49-K017546-022-305-A06C-8409
GenBank Accession LN506672 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K017546-022-305-A06C-8409
ATATTTCCCGGACATGAAGCCATTTACAATTTAAATTGGTTGTAGTTGGCCATGTCCGTT
CTACATATATTAACTGCAGTAATGTTTTGTGATAAATCAGGCAACTAACGGGAGTGGAAT
TAAATGGTTTGAGCGTTAAGGAGTTACAAAACATAGAGAGTCAACTTGAAATGAGTTTAC
GTGGAATTCGTATGAAAAGGGTAATATATGAATGCTCTAGGCCTCTAGCTGGTTCGCATG
ATGCTTAAAAATTGATAATAGAGCATTGAAGTTTAAGCTAATTATTCTTTTCGAACCTTG
CAGGAACAAATTTTGACCAATGAAATTAAAGAGCTAACCAGAAAGGTAAACTATATGATA
TAACACATGATTATTAAAAATCAGACTCGGATGTTGTTGTTTGTGTTTACATTGTAAACA
CACATAATTTGCAGATGTAAAATGCAAACGATATTTTTAGTTTCTTCTTCGGTTTGTGTT
TGCATTTGCAATTTACAAAAAAATCTGTTCTTTGAGAAAAGATACTAAAGATGTTGTGTT
TAAGTTTGAAACGCATTATTAATCAATGTTATCATTTCTAGTTTTCAGGTTCTTATAAGT
GTTTTATCTTTTGCAGAGGAATCTTGTTCATCATGAAAACCTCGAAT

Last Updated on 06.12.2018 16:11