Sequences from confirmation amplicon


Confirmation sequences for line 305F05 and hit At3g13670 are shown.
Line ID 305F05
Locus At3g13670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4469201
Primers used for PCR DL90 - 8474, show primer details
   
Sequence Name 04-K018754-022-305-F05C-DL90
GenBank Accession LN487727 [GenBank]
Sequencing primer gene specific primer, DL90: 5'-AATTATACCCTCTCAGGGAAAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K018754-022-305-F05C-DL90
TATTTCCTTCTTCTTCCTGAGCAGCCGCCTTGTTAGAGCCACCACTATCATTACCAATAG
CCATATCTTTCTTCTCTTCTTCAACGACTACCTCTTTACCCTTGTTTTTATCGATATCGC
TATCCTTAGACGGCACAACAACCGGAATCTCCTGAGGTATCTCTTTATCTTCCTCCTTTA
ATTTCCTCGCCGCTAATCTAGTCCTCGGCCTCTCTGCAACTCCTGTAACTCCGCCGGCAA
CCGGTTTTGGAGCTTTAACGGTACTCTTCTTCGGTTTCTTTTCTGTAGGTGTTTGCGGAA
GCTGATTGGGAGCTACAGCATCAGTAACACGGCCACGGCGGACTCCACGGCGAAGCTCCG
GCATAGCTCAAACCCCATGCAATCGAGACGGGACACGAAGAGTCCGATCATTATAGTCCT
CCGAAAACGAGGGCCTGGGTCATGTCGGATTTGGTAAGCTATTTGATATATAATCGGAGA
TTCGATTTGCCGATGATGAAGATGGCGATTTAGGGTTACGACGGTTCAAGTTTGCGATGA
GAAAAATAGAAGTTTATGGATGAAAGTTCCCAAAATGGAGAGAGAAAAGAAGACNGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAA
   
Sequence Name 08-K018754-022-305-F05C-8409
GenBank Accession LN489520 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K018754-022-305-F05C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCTTCTTTTCTCTCT
CCATTTTGGGAACTTTCATCCATAAACTTCTATTTTTCTCATCGCAAACTTGAACCGTCG
TAACCCTAAATCGCCATCTTCATCATCGGCAAATCGAATCTCCGATTATATATCAAATAG
CTTACCAAATCCGACATGACCCAGGCCCTCGTTTTCGGAGGACTATAATGATCGGACTCT
TCGTGTCCCGTCTCGATTGCATGGGGTTTGAGCTATGCCGGAGCTTCGCCGTGGAGTCCG
CCGTGGCCGTGTTACTGATGCTGTAGCTCCCAATCAGCTTCCGCAAACACCTACAGAAAA
GAAACCGAAGAAGAGTACCGTTAAAGCTCCAAAACCGGTTGCCGGCGGAGTTACAGGAGT
TGCA

Last Updated on 06.12.2018 16:11