Sequences from confirmation amplicon


Confirmation sequences for line 305G09 and hit At3g21520 are shown.
Line ID 305G09
Locus At3g21520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7582488
Primers used for PCR RU77 - 8474, show primer details
   
Sequence Name 87-K027491-022-305-G09C-8409
GenBank Accession LN514706 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K027491-022-305-G09C-8409
CATTTACAATTGAATATATCAAAGCCATGACAAGTGTCTTCTGTGTTTCCCTGAACCGAG
GATAAAAGCAGCTCGCGGTATTAGCGTCAAGCAGCACCAGTGTACCAAACACAGCCAGCA
CGAAACCCGCATGAACGAAATCCGCTATCCTAAGCTTGTACTTGGATAAATCCACGGATC
CTGGCTCCGCGTAAGTCCAAAGACCTTTCTTGGTGACTATACCGAACTTTCTTGATCCGT
CAACGCCTTTGAAACTATCGGTAAAGCATGAGAAGACGCAGGAAAAGCTGCAAAGGGCGA
CAAGAATGCTCGACATGACCTTATTACCTGTGGAGCATTCACCGTCGTTAGTGA
   
Sequence Name 88-K027491-022-305-G09C-RU77
GenBank Accession LN514811 [GenBank]
Sequencing primer gene specific primer, RU77: 5'-ACTTAACCCTGTCCTCACTAACGA-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K027491-022-305-G09C-RU77
TATAGGTCATGTCGAGCATTCTTGTCGCCCTTTGCAGCTTTTCCTGCGTCTTCTCATGCT
TTACCGATAGTTTCAAAGGCGTTGACGGATCAAGAAAGTTCGGTATAGTCACCAAGAAAG
GTCTTTGGACTTACGCGGAGCCAGGATCCGTGGATTTATCCAAGTACAAGCTTAGGATAG
CGGATTTCGTTCATGCGGGTTTCGTGCTGGCTGTGTTTGGTACACTGGTGCTGCTTGACG
CTAATACCGCGAGCTGCTTTTATCCTCGGTTCAGGGAAACACAGAAGACACTTGTCATGG
CTTTGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11