Sequences from confirmation amplicon


Confirmation sequences for line 306H03 and hit At1g71692 are shown.
Line ID 306H03
Locus At1g71692 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26954370
Primers used for PCR PH41 - 8474, show primer details
   
Sequence Name 01-K017518-022-306-H03C-PH41
GenBank Accession LN486374 [GenBank]
Sequencing primer gene specific primer, PH41: 5'-TGTCGAATTACAAATAGTGACCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K017518-022-306-H03C-PH41
ACACACACACACAAACATGAAATACGTATATGCATCAATATATAAAATAATAGTTCAAAG
CGTGTCAATAACTATAGTTTAGGCCTTAGGGCAACCTTTATAAAGTGGTCAGATCAACTT
TTATTAAAAGATTGTGCTGATTATAAACCAATAATACTACTTCATTCCGAAACACAATGC
GATTGAATAACCAGAGATATAAAATATTAATTAAAGTGTAATACATTGTCTCTAAGCATC
TTTTACTTTAACAGTAATTACTTTGACTTGTTTCGTAACTATTTTATGATAGTACAACGT
TCCACAACAATGTCAGTTCCTCTCAGTAGTCAACAAATTAAAAATTCGGAGAATTGGAGA
GCTAGGTGCAATGAATGTTGTACTTCACTAACTATTTTCATGTATAGAATCAGAATCAGG
TGCCCTACCTAACTTCCCTGAGTATTTTAATTTCAAATCAATTAACAACGTCCAACAGAT
TTTCCCTCTGCATGTCAACACGTGTTAGCATGTTGCACACTAAAATAATGTTTATGGAAC
AAAAAGACTAATGAGTAAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATNGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATTTCGACGTGTCTAC
   
Sequence Name 02-K017518-022-306-H03C-8409
GenBank Accession LN486823 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K017518-022-306-H03C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATTTACTCATTAGTCTTTT
TGTTCCATAAACATTATTTTAGTGTGCAACATGCTAACACGTGTTGACATGCAGAGGGAA
AATCTGTTGGACGTTGTTAATTGATTTGAAATTAAAATACTCAGGGAAGTTAGGTAGGGC
ACCTGATTCTGATTCTATACATGAAAATAGTTAGTGAAGTACAACATTCATTGCACCTAG
CTCTCCAATTCTCCGAATTTTTAATTTGTTGACTACTGAGAGGAACTGACATTGTTGTGG
AACGTTGTACTATCATAAAATAGTTACGAAACAAGTCAAAGTAATTACTGTTAAAGTAAA
AGATGCTTAGAGACAATGTATTACACTTTAATTAATATTTTATATCTCTGGTTATTCAAT
CGCATTGTGTTTCGGAATGAAGTAGTATTATTGGTTTATAATCAGCACAATCTTTTAATA
AAAGTTGATCTGACCACTTTATAAAGGTTGCCCTAAGGCCTAAACTATAGTTATTGACAC
GCTTTGAACTATTATTTTATATATTGATGCATATACGTATTTCATGTTTGTGTGTGTGTG
TACCAACATGGGTCACT

Last Updated on 06.12.2018 16:11