Sequences from confirmation amplicon


Confirmation sequences for line 308D07 and hit At1g49780 are shown.
Line ID 308D07
Locus At1g49780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18430031
Primers used for PCR BE54 - 8474, show primer details
   
Sequence Name 74-K028752-022-308-D07C-8409
GenBank Accession LN512989 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K028752-022-308-D07C-8409
CGAACATAGAAGCCATCGCTAAAAGCGCAAACGGCGTCGAAACGGATTCCTACACCTAAA
CAACCGGCGGAATCCAATCTCCGTTAGGTCACTTCTCAGTCAAGCATCCGCAATTACCGG
AACTCACGTCTCTGTTCGTTCACGCGCCGCCGCTATTCGTCGTTTGAGAGGATTAGCTAG
AGATAGTGAGAAGAATCGCGTTTTGATCGCTGGTCATAACGCGAGGGAGATTTTGGTGAG
GATTTTGTTCGCCGATATTGAAACGACGTCGTTGTCTTCGGAGCTTGTTTCTGAGTCGCT
TGCTCTTTTGGTTTTATTGCATATGACGGAGACGGAGTGTGAAGCTGTTGCGTCGGATCC
TAGTCGGGTCGGGTTTATGACCCGATTGTTGTTTGATTCTTCGATGAAAATTA
   
Sequence Name 74-K028752-022-308-D07C-BE54
GenBank Accession LN512990 [GenBank]
Sequencing primer gene specific primer, BE54: 5'-CCCTAATTTCAATCGAAGAATCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K028752-022-308-D07C-BE54
GGATCGACCAACAGCTTCACACTCCGTCTCCGTCATATGCAATAAAACCAAAAGAGCAAG
CGACTCAGAAACAAGCTCCGAAGACAACGACGTCGTTTCAATATCGGCGAACAAAATCCT
CACCAAAATCTCCCTCGCGTTATGACCAGCGATCAAAACGCGATTCTTCTCACTATCTCT
AGCTAATCCTCTCAAACGACGAATAGCGGCGGCGCGTGAACGAACAGAGACGTGAGTTCC
GGTAATTGCGGATGCTTGACTGAGAAGTGACCTAACGGAGATTGGATCCGCCGGTTGTTT
AGGTGTAGGAATCCGTTCGACGCCGTTAGAGCGGTTAGCGATGGCATCTATGTCGACGTA
AATGCTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11