Sequences from confirmation amplicon


Confirmation sequences for line 308E07 and hit At5g63110 are shown.
Line ID 308E07
Locus At5g63110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25318304
Primers used for PCR CK38 - 8474, show primer details
   
Sequence Name 11-K018154-022-308-E07C-CK38
GenBank Accession LN490902 [GenBank]
Sequencing primer gene specific primer, CK38: 5'-AGATCATAAAGAAGGAATGGAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K018154-022-308-E07C-CK38
CATCTTGAGCAACTCCAGAATCCCTAGCACGATGTCGTTTACATAGCAAAACCCAGAAGC
CTCGCTTTTCTTAGCATGGTGAAGCCCACCGCCCCAATTGATAGCGATATCAGCGTCCTG
TCTGTTTAATTTGACGGCAGCACCAATAGAACCTCCGGCGGAAGCACGGCAAAAATCAAA
AAGTCCGTCGAAGACAGGACAATCCTCACCGACATTGAATCGCCTTAGGTTTCGTGCAGC
GGAAGGATCGCCCATAGATTCCGGCGAAACGGAAGCGAGGAAGTCAACATACTCCGGCGA
ATGGAATCGGCCGATATCGGAGGCGTCAGCGAGGCTAGGGCGACTGATTTCTAAGCGACG
GTGGAGGTGATAGTGAATGATTAGGCTATGAGCCATACGGATCCGGTGAGGCTTCATCGG
GTGGCCTTGACCGTAGTAGTAGTCTCCGATCGTCGGCTCGTAGAAGTAACTGACTCGCCG
CTTACGTCCGTCGGGTCCCGACGGCAGAGAGATGCCGCTTTCGTCTGCCTCCATCTCCGT
CTCTCACTCAGAATCTCTCTCTCACTCAGAGTCTCTCTCTATTCCCAAGTTTTACTCAGA
GCANAATTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATG
   
Sequence Name 12-K018154-022-308-E07C-8409
GenBank Accession LN491361 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K018154-022-308-E07C-8409
TAGATTTCCCGGACATGAAGCCATTTACAAATTTTGCTCTGAGTAAAACTTGGGAATAGA
GAGAGACTCTGAGTGAGAGAGAGATTCTGAGTGAGAGACGGAGATGGAGGCAGACGAAAG
CGGCATCTCTCTGCCGTCGGGACCCGACGGACGTAAGCGGCGAGTCAGTTACTTCTACGA
GCCGACGATCGGAGACTACTACTACGGTCAAGGCCACCCGATGAAGCCTCACCGGATCCG
TATGGCTCATAGCCTAATCATTCACTATCACCTCCACCGTCGCTTAGAAATCAGTCGCCC
TAGCCTCGCTGACGCCTCCGATATCGGCCGATTCCATTCGCCGGAGTATGTTGACTTCCT
CGCTTCCGTTTCGCCGGAATCTATGGGCGATCCTTCCGCTGCACGAAACCTAAGGCGATT
CAATGTCGGTGAGGATTGTCCTGTCTTCGACGGACTTTTTGATTTTTGCCGTGCTTCCGC
CGGAGGTTCTATTGGTGCTGCCGTCAAATTAAACAGACAGGACGCTGATATCGCTATCAA
TTGGGGCGGTGGGCTTCACCATGCTAAGAAAAGCGAGGCTTCTGGGTTTTGCTATGTAAA
CGACATCGTGCTAGGGATTCTGGAGTTGCTCAAGATGTTTAAGGTAAATGCTCCATTC

Last Updated on 06.12.2018 16:11