Sequences from confirmation amplicon


Confirmation sequences for line 308H02 and hit At1g74030 are shown.
Line ID 308H02
Locus At1g74030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27841953
Primers used for PCR BG43 - 8474, show primer details
   
Sequence Name 92-K101673-0022-308-H02C-8409
GenBank Accession LN515182 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K101673-0022-308-H02C-8409
GAGCCATTTACATTGAAGCGGAGAATAAAATGATGTTATATAATTTGAAAAACGTCCTTT
TGGAGGTTGGTGCAGGCGGAGGCGATGAAAACTAAGGAGTCAAAAGAACCATACTTAAAC
CGGTTCATTTTCCAATTGGTTGACTGTTAAAGAGAAGTTTCTGGTTTTGGTTTGGTTCGG
TTCGGTTCGGAACAATTCAAATAAATAAAACAAATCAAAAATATTCACTAGCAAAGTAGT
AACCAGAGACACTGTGCCGTCGCCCGTCGCCGCCGCCGCCACACTATCATCTCTCTCAGG
TTTTTGATTTTCCACGGCAATGGAGTCTTCGATTCTCAAAAGCCCTAATCTCTCTTCACC
ATCGTTCGGTGTACCTTCAATTCCCGCCTTATCCTCCTCCTCCACCTCACCATTTTCATC
TCTTCATCTCCGATCACAGAACCACCGTACC
   
Sequence Name 92-K101680-0022-308-H02C-BG43
GenBank Accession LN515183 [GenBank]
Sequencing primer gene specific primer, BG43: 5'-TAAGAGAGATGGTACGGTGGTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K101680-0022-308-H02C-BG43
AAATAATGGTGAGGTGGAGAGAGGATAAGGCGGGAATTGAAGGTACACCGAACGATGGTG
AAGAGAGATTAGGGCTTTTGAGAATCGAAGACTCCATTGCCGTGGAAAATCAAAAACCTG
AGAGAGATGATAGTGTGGCGGCGGCGGCGACGGGCGACGGCACAGTGTCTCTGGTTACTA
CTTTGCTAGTGAATATTTTTGATTTGTTTTATTTATTTGAATTGTTCCGAACCGAACCGA
ACCAAACCAAAACCAGAAACTTCTCTTTAACAGTCAACCAATTGGAAAATGAACCGGTTT
AAGTATGGTTCTTTTGACTCCTTAGTTTTCATCGCCTCCGCCTGCACCAACCTCCAAAAG
GACGTTTTTCAAATTATATAACATCATTTTATTCTCCGCTTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11