Sequences from confirmation amplicon


Confirmation sequences for line 309A01 and hit At1g03740 are shown.
Line ID 309A01
Locus At1g03740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:935650
Primers used for PCR DL57 - 8474, show primer details
   
Sequence Name 05-K018514-022-309-A01C-DL57
GenBank Accession LN488154 [GenBank]
Sequencing primer gene specific primer, DL57: 5'-TAGCAGATTTTGGGTTAGCAACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K018514-022-309-A01C-DL57
AGTGTTTCATTGACTAGCCATGTTGTCACTCTTTGGTACCGGCCACCAGAACTTTTGCTT
GGAGCCTCTCATTACGGTGTTGGGGTTGATTTATGGAGCACAGGTTGCATCTTAGGTGAA
TTATATGCCGGTAAACCGATCCTCCCTGGAAAAACCGAGGTATGCAAATTCTCGTAGATT
TCATTTCCTCCAAATTCAGGTTGCATCTTGTTTGCTTCAACTCAAATGTAGCCCTTAATC
TTTCTAGGTAGAACAATTGCATAAAATCTTCAAGCTTTGCGGTTCACCAACAGAAAATTA
CTGGAGAAAACAGAAGTTACCATCTTCAACAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 06-K018514-022-309-A01C-8409
GenBank Accession LN488600 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K018514-022-309-A01C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTTGAAGATG
GTAACTTCTGTTTTCTCCAGTAATTTTCTGTTGGTGAACCGCAAAGCTTGAAGATTTTAT
GCAATTGTTCTACCTAGAAAGATTAAGGGCTACATTTGAGTTGAAGCAAACAAGATGCAA
CCTGAATTTGGAGGAAATGAAATCTACGAGAATTTGCATACCTCGGTTTTTCCAGGGAGG
ATCGGTTTACCGGCATATAATTCACCTAAGATGCAACCTGTGCTCCATAAATCAACCCCA
ACACCGTAATGAGAGGCTCCAAGCAAAAGTTCTGGTGGCCGGTACCAAAGAGTGACAACA
TGGCTAGTCAATGAAACACTTTTTGCAGGGTCGAAAAAAGTTGCTAACCCAANATCTGCT

Last Updated on 06.12.2018 16:11