Sequences from confirmation amplicon


Confirmation sequences for line 309E09 and hit At1g66410 are shown.
Line ID 309E09
Locus At1g66410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24774963
Primers used for PCR RC01 - 8474, show primer details
   
Sequence Name 31-K021852-022-309-E09C-8409
GenBank Accession LN499977 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K021852-022-309-E09C-8409
TTATGGAAACTCTGATCTGTTCTAAAACATACATGACTATACATGACTGTTTGCTTCTCA
TACGAAACTAAATATTATCAGAGAACTTAAACCTGTAGCTACATACCACCTTACAAGTAG
ATTCCCATAACAAAGTCAGATTGATATACATACGAAACATGAATGAAACCAGGTCTTTTG
TTTAAACGTTTTTGAGCAAAAAAAAGCCACTAATCAACCAAAAACATGACCATTAAATGA
ATCTCATAAAAACACTAGAATCTGAATATCTTTGGCTATGGAAAAAGCATGACAGGTGCT
AAAACTAAACAGTATCTTTGAACTGAAATCTATAGCAATATACCTACTTAAACTACAGAA
GAATGACACAAAAAGACGATTCAGAACAAGAATCAGGAACATGATATTCTGCACAAAGCT
AGTAACATTTCAATATAAGTCACCTTCAAAGAAACATCAAAATCACAAATCCATGCTTAG
AGATGGAACAAAACTACATCAAAAGAGACAAT
   
Sequence Name 32-K021852-022-309-E09C-RC01
GenBank Accession LN500404 [GenBank]
Sequencing primer gene specific primer, RC01: 5'-TGATTGAATCGTTTGATTGTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K021852-022-309-E09C-RC01
TCTCTAGCATGGATTTGTGATTTTGATGTTTCTTTGAAGGTGACTTATATTGAAATGTTA
CTAGCTTTGTGCAGAATATCATGTTCCTGATTCTTGTTCTGAATCGTCTTTTTGTGTCAT
TCTTCTGTAGTTTAAGTAGGTATATTGCTATAGATTTCAGTTCAAAGATACTGTTTAGTT
TTAGCACCTGTCATGCTTTTTCCATAGCCAAAGATATTCAGATTCTAGTGTTTTTATGAG
ATTCATTTAATGGTCATGTTTTTGGTTGATTAGTGGCTTTTTTTTGCTCAAAAACGTTTA
AACAAAAGACCTGGTTTCATTCATGTTTCGTATGTATATCAATCTGACTTTGTTATGGGA
ATCTACTTGTAAGGTGGTATGTAGCTACAGGTTTAAGTTCTCTGATAATATTTAGTTTCG
TATGAGAAGCAAACAGTCATGTATAGTCATGTATGTTTTAGAACAGATCATGAGTTTTCC
ATAATGAAAGATTCCAGAGTCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
Primers used for PCR FN65 - 8474, show primer details
   
Sequence Name 09-K035333-022-309-E09C-8409
GenBank Accession LN490080 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K035333-022-309-E09C-8409
TCCGGACTGAGCCATTTACATTGAATATATCCTGTAGCAGAGTCTAGTGCTTTTTTTTTC
TGGCATCCATTGATGGTCATGTGTTTAGCTGTTTGTTGGCTCTTGTAGCTTAAAAAAGTT
TTATTGGAGACTTATTTATCACAAGACCTGGTTTTTATTCATGTTATCTTTTACTGATTG
AACAGGTTGCATCACCACAAAGGAGCTGGGAACAGTGATGCGGTCACTAGGGCAGAACCC
AACAGAAGCTGAGCTACAAGACATGATCAACGAGGTTGACGCTGATGGAAACGGAACCAT
TGACTTCCCTGAGT
   
Sequence Name 09-K035333-022-309-E09C-FN65
GenBank Accession LN490081 [GenBank]
Sequencing primer gene specific primer, FN65: 5'-TAAGAACTCAGGGAAGTCAATGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K035333-022-309-E09C-FN65
GTCACCTCGTTGATCATGTCTTGTAGCTCAGCTTCTGTTGGGTTCTGCCCTAGTGACCGC
ATCACTGTTCCCAGCTCCTTTGTGGTGATGCAACCTGTTCAATCAGTAAAAGATAACATG
AATAAAAACCAGGTCTTGTGATAAATAAGTCTCCAATAAAACTTTTTTAAGCTACAAGAG
CCAACAAACAGCTAAACACATGACCATCAATGGATGCCAGAAAAAAAAAGCACTAGACTC
TGCTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
CTTTATTTCGACGTGTCTACATTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11