Sequences from confirmation amplicon


Confirmation sequences for line 311A11 and hit At1g12120 are shown.
Line ID 311A11
Locus At1g12120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4117715
Primers used for PCR FA89 - 8474, show primer details
   
Sequence Name 84-K037327-022-311-A11C-FA89
GenBank Accession LN514389 [GenBank]
Sequencing primer gene specific primer, FA89: 5'-ATCCAAAACACAAAAATCTTCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K037327-022-311-A11C-FA89
AGCATGCAAATAAGTAAAATTATTCAGAAGATGATTCCATAAAAAGAAACAATCGCACAT
ATATTCACATTTTGATGATAGATATATAAACATATTCCTAAATCCTGAATTCAACAGAAG
AACTATAAGAGGAAAAGTAAAAGCATCAACTTCATGACGAACATAAAACATAGTACTACT
TCGACTTTACATAAAAAAAACTATAAAAAGAATAACAATCTATAAATATTTTACTGTAAA
TTAAGGAATTACCCAGATAGAGAAAAAAAGGAAGCTTGAAGCTTTCCAATGGTAAGATTG
ACGAACAGATCTGGGAGACGAGAACAGGAGAAAGAAATGAAAAATATCTCAAGAAGGTGT
GAGATCCATGGTCAGATTCCAATGGTTATTGTCTCTATCAATATACACACAAATAACGTG
TATCTGTAATTATCACATAAATTTTTTATAAATTATATAAAAAATCGGATCATGAGGAGC
GATATAGAGAGAGAAGAGAGAGGAGACTCTCTCAAATCTCAGAAAATACAGAGAGAGAAA
ATGGTCTCTGAGTGGTTAATGTGCAACATCAGCTATCTTTTTCTGTGATTTATAATATTT
TAACTTTATAAGAAAAAAACAAATTTATTATTATTACTAATTTGACAAAAGATTTTTAGG
TGTGATTTTTTTTCCTCAATTTAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACNAATTACGATCCGTCGTATTTATAGGCGAAACAATAAACAAATTATT
CTAATTC
   
Sequence Name 91-K037327-022-311-A11C-8409
GenBank Accession LN515093 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K037327-022-311-A11C-8409
ACTTTTCCTCTTATAGTTCTTCTGTTGAATTCAGGATTTAGGAATATGTTTATATATCTA
TCATCAAAATGTGAATATATGTGCGATTGTTTCTTTTTATGGAATCATCTTCTGAATAAT
TTTACTTATTTGCATGCTTTTAGAGTTTTATTTATAAGCAGAAGCCTGAAGATTTTTGTT
TTTTT

Last Updated on 06.12.2018 16:11