Sequences from confirmation amplicon


Confirmation sequences for line 312C03 and hit At3g46010 are shown.
Line ID 312C03
Locus At3g46010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16910490
Primers used for PCR VL30 - 8474, show primer details
   
Sequence Name 41-K021737-022-312-C03C-8409
GenBank Accession LN503922 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K021737-022-312-C03C-8409
AGCCATTTACAATTAGAATCAAAACTGTGCGAGAGAGCTTCTCTGTTGAGTTCACTGTGG
CTCTTTCGATTCCTCTATCTAAAATTTGAAATCAGAAGAGTATTTACGGCTTCCTGTTAT
TGCAAAAAATTTCAAAGTTATATATCAATTTCGATAACATTTGGTTGTTTGATCCATTAT
CCTTGATGTTTGATTCATCAGTTTCATGTAATTGATAATCACTCTAGAGGTTTGAGAACA
ATAGAGGTTTTTGGATTGTGTTATGTGAAAAGATTTGAATATGAGTGTTGTTTGGTTTAA
CAGGCGAACGCGGCATCTGGAATGGCTGTGCATGATGATTGCAAGCTGAGATTTCTGGAA
CTGAAGGCTAAAAGGACACACCGTTTCATAGTTTACAAGATTGAGGAGAAGCAGAAGCAA
GTTGTTGTTGAGAAAGTTGGTCAACCGATCCAAACTTACGAGGAGTTTGCAGCATGTCTT
CCAGCTGATGAATGCCGTTACGCCATTTACGATTTTGACTTTGTAACTGCTGAGAATTGC
CAGAAGAGCAAGATCTTCTTCATCGCGTGGTAGCTAGTCACATAATCTGCCATTAAATCT
GTGTCTCGTTTTATTGTTATCTTCCAAA
   
Sequence Name 42-K021737-022-312-C03C-VL30
GenBank Accession LN504311 [GenBank]
Sequencing primer gene specific primer, VL30: 5'-AAGTACGTTGAAAACCTTTGGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K021737-022-312-C03C-vl30
GATTTATGGCAGATATGTGACTAGCTACCACGCGATGAAGAAGATCTTGCTCTTCTGGCA
ATTCTCAGCAGTTACAAAGTCAAAATCGTAAATGGCGTAACGGCATTCATCAGCTGGAAG
ACATGCTGCAAACTCCTCGTAAGTTTGGATCGGTTGACCAACTTTCTCAACAACAACTTG
CTTCTGCTTCTCCTCAATCTTGTAAACTATGAAACGGTGTGTCCTTTTAGCCTTCAGTTC
CAGAAATCTCAGCTTGCAATCATCATGCACAGCCATTCCAGATGCCGCGTTCGCCTGTTA
AACCAAACAACACTCATATTCAAATCTTTTCACATAACACAATCCAAAAACCTCTATTGT
TCTCAAACCTCTAGAGTGATTATCAATTACATGAAACTGATGAATCAAACATCAAGGATA
ATGGATCAAACAACCAAATGTTATCGAAATTGATATATAACTTTGAAATTTTTTGCAATA
ACAGGAAGCCGTAAATACTCTTCTGATTTCAAATTTTAGATAGAGGAATCGAAAGAGCCA
CAGTGAACTCAACAGAGAAGCTCTCTCGCACAGTTTTGATTCTAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCT

Last Updated on 06.12.2018 16:11