Sequences from confirmation amplicon


Confirmation sequences for line 312E06 and hit At5g66700 are shown.
Line ID 312E06
Locus At5g66700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26635488
Primers used for PCR CC06 - 8474, show primer details
   
Sequence Name 27-K023369-022-312-E06C-8409
GenBank Accession LN498204 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K023369-022-312-E06C-8409
GTAGTTAACTATGGTATTAACTAATGATGTATTTTTTTGCTGTTGTAGATATTGAAACTA
ACAGAACAATTGAGTGAAGCTCAAAGTGAAATTCGAAAACTGTCGGAACGACTTGAAGAA
ATGCCAACCAACAGTTCAAGTTCATCGCTTTCTGTTGAAGCCAACAATGCACCAACTGAT
TTCGAGCTTGCCCCGGAAACTAATTATAACATCCCGTTTTATATGTTAGATAATAATTAT
TTACAAAGCATGGAGTATTGGGATGGTTTGTATGTATAATCAGTTTATCAACTAAATATC
TCGTGCTTTATTGATGCAACTCATGTTATTATAAGTAATGTAGAAATAGGTGTTGGTGAA
ACGGTTGTAATTATGTGTTGTTTTCAGTATTTATTTTAATATATTACTTTGTTTATTATC
ACTTTGCTTCTTCTAGTTCCATATATATAAATCTTTCAAAAAAAAAAAAGGTAACATATA
TGGATATTTGCTCATTTATCAAAAGATATTATTAATGTTCAGTATACAATTAATTAGTTA
CCAAACAGTTGTTATTTATATC
   
Sequence Name 28-K023369-022-312-E06C-CC06
GenBank Accession LN498673 [GenBank]
Sequencing primer gene specific primer, CC06: 5'-AAATCAAGAGTTTCTATGTCGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K023369-022-312-E06C-CC06
GGGATGAGGCTATAAATAACAACTGTTTGGTAACTAATTAATTGTATACTGAACATTAAT
AATATCTTATGATAAATGAGCAAATATCAATATATGTTACCTTTTTTTTTTTTGAAAGAT
TTATATATATGGAACTAGAAAAAGCAAAGTGATAATAAACAAAGTAATATATTAAAATAA
ATACTGAAAACAACCCATAATTACAACCGTTTCCCCAACCCCTATTTCTACATTACTTAT
AAAAACATGGGTTGCATCAATAAAGCCCGAGATATTTAGTTGATAAACTGATTATACATA
CAAACCATCCCAATACTCCAGGCTTTGTAAATAATTATTATCTAACATATAAAACGGGAT
GTTATAATTAGTTTCCGGGGCAAGCTCAAAATCAGTTGGGGCATTGTTGGCTTCAACAAA
AAGCGATGAACTTGAACTGTGGGTGGGCATTTCTTCAAGTCGTTCCGACGGTTTTCGAAT
TTCACTTTGAGCTTCCCCCAATTGTTCTGTTAGTTTCAATATCTCCAACGGCAAAAAAAT
ACCTCATTAGTTAATCCCATAGTTAA

Last Updated on 06.12.2018 16:11