Sequences from confirmation amplicon


Confirmation sequences for line 313G08 and hit At4g21330 are shown.
Line ID 313G08
Locus At4g21330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11350548
Primers used for PCR PH71 - 8474, show primer details
   
Sequence Name 03-K018600-022-313-G08C-PH71
GenBank Accession LN487281 [GenBank]
Sequencing primer gene specific primer, PH71: 5'-TTGGTGATGATTGTTGTTGTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K018600-022-313-G08C-PH71
TACTAAAGCAAGTATTGTTGAAGATGCGATTACTTACATAGGAGAGCTTCAAAACAATGT
TAAGAATCTCTTAGAGACATTTCATGAAATGGAAGAAGCTCCTCCTGAGATTGATGAAGA
ACAAACGGATCCAATGATAAAACCTGAAGTTGAAACTAGTGATCTTAACGAAGAGATGAA
GAAACTCGGAATCGAGGTTTTTAAAATTTCTCTAATCGATTTTTGATTCATTTTCTTTAT
CTTTCTTGATGATTTTTTATTTTTGGGACAGGAGAATGTGCAATTGTGTAAGATTGGGGA
GAGGAAGTTTTGGTTAAAGATCATAACAGAGAAGAGAGATGGGATCTTTACTAAATTCAT
GGAGGTTATGAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGT
GTCTACAT
   
Sequence Name 04-K018600-022-313-G08C-8409
GenBank Accession LN487724 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K018600-022-313-G08C-8409
TTTCCCGGACATGAAGCCATTCATAACCTCCATGAATTTAGTAAAGATCCCATCTCTCTT
CTCTGTTATGATCTTTAACCAAAACTTCCTCTCCCCAATCTTACACAATTGCACATTCTC
CTGTCCCAAAAATAAAAAATCATCAAGAAAGATAAAGAAAATGAATCAAAAATCGATTAG
AGAAATTTTAAAAACCTCGATTCCGAGTTTCTTCATCTCTTCGTTAAGATCACTAGTTTC
AACTTCAGGTTTTATCATTGGATCCGTTTGTTCTTCATCAATCTCAGGAGGAGCTTCTTC
CATTTCATGAAATGTCTCTAAGAGATTCTTAACATTGTTTTGAAGCTCTCCTATGTAAGT
AATCGCATCTTCAACAATACTTGCTTTAGTCATCTGAAAACGAAACAAAAACAACAACAA
TCATCACC

Last Updated on 06.12.2018 16:11