Sequences from confirmation amplicon


Confirmation sequences for line 313H09 and hit At3g18890 are shown.
Line ID 313H09
Locus At3g18890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6514458
Primers used for PCR DL69 - 8474, show primer details
   
Sequence Name 48-K018602-022-313-H09C-DL69
GenBank Accession LN506371 [GenBank]
Sequencing primer gene specific primer, DL69: 5'-CAACCTGCAATTAAGAAGAAGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K018602-022-313-H09C-DL69
CAATTCTCGTCTCACATAGCTAATGATTGGTGACTGGAGATGGAAGTGGTGATGTTGGAG
GCTTCATGTCCGCGTACCTGCAAAAACATTTCAGTCTTTCAGGTCGCAAAAGAAGAAGTA
ATAACAATGGACACACTTAGAACTAGCATGCTTCTCTCTATTCTCACATTGTGTAAGGTG
ATAAAGGCCTTGGCTTTGGCTGAGCTGTATTGTCTCCTGAAGCAAGGGAAGTGTCTATCG
CACTGCTAACAACAGGTACTGCTTCCGGTGTAGAAGGTGGAATTGTGTTCCGGTGTAGAA
GGTGGATCGTAATTTCCGGTGTACTGATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 49-K018602-022-313-H09C-8409
GenBank Accession LN506678 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K018602-022-313-H09C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATCAGTACACCGGAAATTACGATCCA
CCTTCTACACCGGAACACAATTCCACCTTCTACACCGGAAGCAGTACCTGTTGTTAGCAG
TGCGATAGACACTTCCCTTGCTTCAGGAGACAATACAGCTCAGCCAAAGCCAAGGCCTTT
ATCACCTTACACAATGTGAGAATAGAGAGAAGCATGCTAGTTCTAAGTGTGTCCATTGTT
ATTACTTCTTCTTTTGCGACCTGAAAGACTGAAATGTTTTTGCAGGTACGCGGACATGAA
GCCTCCAACATCACCACTTCCATCTCCAGTCACCAATCATTAGCTATGTGAGACGAGAAT
TGGCCTCCTAAACCCTGGAGCTTCTTCTTAATTGCAGGTT

Last Updated on 06.12.2018 16:11