Sequences from confirmation amplicon


Confirmation sequences for line 314D02 and hit At1g77920 are shown.
Line ID 314D02
Locus At1g77920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29298661
Primers used for PCR MJ03 - 8474, show primer details
   
Sequence Name 43-K017280-022-314-D02C-MJ03
GenBank Accession LN504664 [GenBank]
Sequencing primer gene specific primer, MJ03: 5'-TCTTAAAGATGCAAGTTGTGTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K017280-022-314-D02C-MJ03
CTCATCATCTCTATATATATCCTCTACATATCAAACCAATCATAGTTGGTAATTTAGAAT
CTTTCAAAAACATGCACAAGAATAGATCAAAGCAGAAAGAAAAAAGAAAGTTTTGTGTTG
CCTCACCTTCAGTTATAGATCCAATAGCTGGCTCTTAAAAATTGCATTTGCTCTCACTGA
TACAAACCATAAACAGAATAAACTCTTAATCATTTTTAAAACTAATATAAAGTTTGGAAC
TAAAAAAAACACACGAGAAACTCCAAATTCTCCAAGATCCAAACGTTTCAAGTGAAAAAC
AGAGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT
CTACATTCACG
   
Sequence Name 44-K017280-022-314-D02C-8409
GenBank Accession LN505024 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K017280-022-314-D02C-8409
TGTAGATTTCCCGGACATGAAGCCATTTACTCTCTGTTTTTCACTTGAAACGTTTGGATC
TTGGAGAATTTGGAGTTTCTCGTGTGTTTTTTTTAGTTCCAAACTTTATATTAGTTTTAA
AAATGATTAAGAGTTTATTCTGTTTATGGTTTGTATCAGTGAGAGCAAATGCAATTTTTA
AGAGCCAGCTATTGGATCTATAACTGAAGGTGAGGCAACACAAAACTTTCTTTTTTCTTT
CTGCTTTGATCTATTCTTGTGCATGTTTTTGAAAGATTCTAAATTACCAACTATGATTGG
TTTGATATGTAGAGGATATATATAGAGATGATGAGTTCTTCTTCTCCAACACAACTTGCA
TCTTTAAG

Last Updated on 06.12.2018 16:11