Sequences from confirmation amplicon


Confirmation sequences for line 316D04 and hit At3g25530 are shown.
Line ID 316D04
Locus At3g25530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9272819
Primers used for PCR MJ61 - 8474, show primer details
   
Sequence Name 19-K029910-022-316-D04C-8409
GenBank Accession LN494633 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K029910-022-316-D04C-8409
GCCATTTACAACTCATTATCCTTGCTGCTGGTGACAAGGTACAACTCAAAACTGTACTTA
TGGATTGATTGATAGATCTCAAGAACTGTTTTAGTTGGACTTAGTAGGAAGGAGCTCTCG
ATGTGCGGCTATAATCCGTTTGTGTTTTAATCCTTTTTTGTAAGGCACTCTTTGAGGAAT
CAATCCCAGCTTTTGATGTCTTGGGGAAGAGATCGTTTTACTTGGGACAAGTTGGAAACG
GAGCTAAAATGAAGCTAATAGTGAACATGATAATGGGAAGGTGAATGTCCCGTCTCTTTT
ACAATTACTACCATTAGTAGTAGGAATGGAACATGGCTTCATGATCTTATTGTTTTTCGT
CTGATACAGCATGATGAATGCATTCTCTGAGGGGCTTGTATTGGCTGACAAGAGTGGACT
TAGCTCTGACACTCTTTTGGATATTCTGGTGAGGTGATCAAACTTTTGCAAGCTCTGAAA
TAATGGTGTTGGTTTGAATCGGTTTCTGCTATGGGCAGGATCTGGGAGCAATGACTAACC
CGATGTTCAAGGGGAAAGGACCTTCAATGAACAAGAGTAGTTACCCACCAGCATTTCCAT
TGAAACATCAGCAGAAAGACAT
   
Sequence Name 19-K029910-022-316-D04C-MJ61
GenBank Accession LN494634 [GenBank]
Sequencing primer gene specific primer, MJ61: 5'-AAGAGCTAGCCTCATGTCTTTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K029910-022-316-D04C-MJ61
GGGTACTACTCTTGTTCATTGAAGGTCCTTTCCCCTTGAACATCGGGTTAGTCATTGCTC
CCAGATCCTGCCCATAGCAGAAACCGATTCAAACCAACACCATTATTTCAGAGCTTGCAA
AAGTTTGATCACCTCACCAGAATATCCAAAAGAGTGTCAGAGCTAAGTCCACTCTTGTCA
GCCAATACAAGCCCCTCAGAGAATGCATTCATCATGCTGTATCAGACGAAAAACAATAAG
ATCATGAAGCCATGTTCCATTCCTACTACTAATGGTAGTAATTGTAAAAGAGACGGGACA
TTCACCTTCCCATTATCATGTTCACTATTAGCTTCATTTTAGCTCCGTTTCCAACTTGTC
CCAAGTAAAACGATCTCTTCCCCAAGACATCAAAAGCTGGGATTGATTCCTCAAAGAGTG
CCTTACAAAAAAGGATTAAAACACAAACGGATTATAGCCGCACATCGAGAGCTCCTTCCT
ACTAAGTCCAACTAAAACAGTTCTTGAGATCTATCAATCAATCCATAAGTACAGTTTTGA
GTTGTACCTTGTCACCAGCAGCAAGGATAATGAGTTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11