Sequences from confirmation amplicon


Confirmation sequences for line 316H10 and hit At4g27800 are shown.
Line ID 316H10
Locus At4g27800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13854198
Primers used for PCR DL66 - 8474, show primer details
   
Sequence Name 11-K018613-022-316-H10C-DL66
GenBank Accession LN490905 [GenBank]
Sequencing primer gene specific primer, DL66: 5'-CTGATGATCATTACAGTGGCTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K018613-022-316-H10C-DL66
CCTGATTCATCTTCTTCGTCACCGTTAGCTTCTAGCCTGGATCCAAACGCATGATGAATC
ACTTCATTTCACTCAAAATCATCAAAATTCATTCAAGCTCTGAGAAATTTAAGCTCATTT
TCACCATTTGAGCAAATTGCGGTCAACGCTTTCAAAGGCCTTAATCAAAGCTTCCTTGAT
TGCTGCGAAATCGCCTCCATTCAACAACGATCCAGCTTGCAATGCACCAACACACTCTTT
ATACAGCTCTTCCCTATCAATCATCATCACCATCGTCAACACTTAAACCCTAGAAGACAG
AGCTTGCATGAGAAGCAACACAAGTTACCTGAGGAATTTGACAGAGGACGACCCAGCGTG
GCCGTCGAAGACCGCTGCGTAGGAGAAGCTATCGACAGCATCAGAACGGATTACGATGTC
GTCTTCCATCTCATCTCGGAATCCTTGAACACTCGTGTAACCCCAACGAATCGGCGTCAC
GCCAGTCAGAGACGACNGAGCGTCGATCGCAATCGCGGAGCAACGGTGACGACCATACGT
GGGATAGACGACNAGGCCACCGCCNGCG
   
Sequence Name 12-K018613-022-316-H10C-8409
GenBank Accession LN491364 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K018613-022-316-H10C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTCAGATATTTTTTTACGA
AAATCCACGGCCACTAACCGTAAAGGACACTCTTTTGATTTTTCTCTTCTTCTACTTAAC
TCGCTTTGCCTGACTCACTGAGTCATGGCGCTTCTGAGGCCGCATCTTCATCGCTTTCAC
TCCAACACTCTCCGCCACTCAGCCTATCCTTCCGCCGACGCTGGCGGTGGCCTTGTCGTC
TATCCCACGTATGGTCGTCACCGTTGCTCCGCGATTGCGATCGACGCTCCGTCGTCTCTG
ACTGGCGTGACGCCGATTCGTTGGGGTTACACGAGTGTTCAAGGATTCCGAGATGAGATG
GAAGACGACATCGTAATCCGTTCTGATGCTGTCGATAGCTTCTCCTACGCAGCGGTCTTC
GACGGCCACGCTGGGTCGTCCTCTGTCAAATTCCTCAGGTAACTTGTGTTGCTTCTCATG
CAAGCTCTGTCTTCTAGGGTTTAAGTGTTGACGATGGTGATGATGATTGATAGGGAAGAG
CTGTATAAAGAGTGTGTTGGTGCATTGCAAGCTGGATCGTTGTTGAATGGAGGCGATTTC
GCAGCAATCAAGGAAGCTTTGATTAAGGCCTTTGAAAGCGTTGACCGCAATTTGCTCAAA
TGGTGAAAATGAGCTTAAATTTCTCAGAGCTTGAATGAATTTTGATG

Last Updated on 06.12.2018 16:11