Sequences from confirmation amplicon


Confirmation sequences for line 318C08 and hit At5g54750 are shown.
Line ID 318C08
Locus At5g54750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22243471
Primers used for PCR HF06 - 8474, show primer details
   
Sequence Name 13-K023618-022-318-C08C-8409
GenBank Accession LN491857 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K023618-022-318-C08C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATCATCCTTGTAAGGATATTCCTCA
GCGACTTGTTTCAGTAGCTTTACTTGCAACTCATAAGCATCATCTCCTCGAAGAACATCA
CGCGTCCATGTCACTTCTGTCTTCATTGAGACCTGATCATTGTGCAGACAAAAAATAAGA
ACATAAAACACTGAGCACAAACAAGTTCTGCACTTGATAATGGAGAAGGAGACTGACCAT
TTCCAAAGCTCCTCTAATGACTCCACTTAAGACATTGCAGTAGTAAAGACCTTGGCAAGT
ATCGGGAAGCTCGACGAAATCAACTAGGGGATTGTCCTCTAAGATTATACTGCAGCAAGT
CCCGTCCGAGTCCCAGCTTGTCACTGATGCAGTGACTCCTAAGAACATCTTGAAACCCAC
CTGTGCAATAAGGCAAAGCCCTGTTACTGCTTTCTCAATACCATGAAACC
   
Sequence Name 14-K023618-022-318-C08C-HF06
GenBank Accession LN492326 [GenBank]
Sequencing primer gene specific primer, HF06: 5'-ATCACTCCTTTGGTTTCATGGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K023618-022-318-C08C-HF06
CAGTAACAGGCTTTGCCTTATTGCACAGGTGGGTTTCAAGATGTTCTTAGGAGTCACTGC
ATCAGTGACAAGCTGGGACTCGGACGGGACTTGCTGCAGTATAATCTTAGAGGACAATCC
CCTAGTTGATTTCGTCGAGCTTCCCGATACTTGCCAAGGTCTTTACTACTGCAATGTCTT
AAGTGGAGTCATTAGAGGAGCTTTGGAAATGGTCAGTCTCCTTCTCCATTATCAAGTGCA
GAACTTGTTTGTGCTCAGTGTTTTATGTTCTTATTTTTTGTCTGCACAATGATCAGGTCT
CAATGAAGACAGAAGTGACATGGACGCGTGATGTTCTTCGAGGAGATGATGCTTATGAGT
TGCAAGTAAAGCTACTGAAACAAGTCGCTGAGGAATATCCTTACAAGGATGATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
AT

Last Updated on 06.12.2018 16:11