Sequences from confirmation amplicon


Confirmation sequences for line 318C09 and hit At3g27080 are shown.
Line ID 318C09
Locus At3g27080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9986610
Primers used for PCR DL74 - 8474, show primer details
   
Sequence Name 05-K018603-022-318-C09C-DL74
GenBank Accession LN488156 [GenBank]
Sequencing primer gene specific primer, DL74: 5'-CTTTAGCAAGTGTGAAGAATGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K018603-022-318-C09C-DL74
TAATACTAAGCCTAAGGACTAATGATTATGATTACAAGGATGCTTGAAACATTAACAACC
AAGTTCATATGGGAGTAAAGAGATCTAAAGATGAATAAGATTCTTATACCAAATTCAATC
ATATTAAAACAGAGTATAGCTTTAAAGAACCTTGAATCATTTGCTTTGCATCTGAGATGC
TATGAAACTGAGATAACTCGAGTAAAACTCCTCCCCATCTAGTCAGATTCTGCAACAACA
AGAACAAAAAATCAAAAAGATTCGTTTTTTTCTTTCTGCCATCGAATCACAAAGCAGAGA
TCAGATCGAACTGAATCTGAAATCATGAAATGAAGAGAAAACTAACATCGGCATCTAAAG
GATTTGACTTGTAGGTATTTTCGGCGTCTTGACGAATTTGTTCGAACAATAGTATCCTAT
CGAACTCAGTTTCCGTATCCATGGCTTCTCAAAGCTTTTCTTCTTTCGTACAAAACTTTT
GGCTTTTCTTCTACAATTGTAAACAGCTTATGTCCCCGGGTACATAATCGCCTTGCAGCA
CATTTTGATCAGCAATGATACATGGATTGCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATNGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACA
   
Sequence Name 06-K018603-022-318-C09C-8409
GenBank Accession LN488602 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K018603-022-318-C09C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCAATCCATGTA
TCATTGCTGATCAAAATGTGCTGCAAGGCGATTATGTACCCGGGGACATAAGCTGTTTAC
AATTGTAGAAGAAAAGCCAAAAGTTTTGTACGAAAGAAGAAAAGCTTTGAGAAGCCATGG
ATACGGAAACTGAGTTCGATAGGATACTATTGTTCGAACAAATTCGTCAAGACGCCGAAA
ATACCTACAAGTCAAATCCTTTAGATGCCGATGTTAGTTTTCTCTTCATTTCATGATTTC
AGATTCAGTTCGATCTGATCTCTGCTTTGTGATTCGATGGCAGAAAGAAAAAAACGAATC
TTTTTGATTTTTTGTTCTTGTTGTTGCAGAATCTGACTAGATGGGGAGGAGTTTTACTCG
AGTTATCTCAGTTTCATAGCATCTCAGATGCAAAGCAAATGATTCAAGGTTCTTTAAAGC
TATACTCTGTTTTAATATGATTGAATTTGGTATAAGAATCTTATTCATCTTTAGATCTCT
TTACTCCCATATGAACTTGGTTGTTAATGTTTCAAGCATCCTTGTAATCATAATCATTAG
TCCTTAGGCTTAGTATTATGATTCAGCATTCTT

Last Updated on 06.12.2018 16:11