Sequences from confirmation amplicon


Confirmation sequences for line 318G08 and hit At4g01520 are shown.
Line ID 318G08
Locus At4g01520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:659300
Primers used for PCR GC66 - 8474, show primer details
   
Sequence Name 42-K033708-022-318-G08C-8409
GenBank Accession LN504368 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K033708-022-318-G08C-8409
GCCATTTACAATTGAAAAAGAAAGAAAAACACAGAGAGAGAGAAGCCGAAAAAACTTCAC
TCAAACCCTAATCCCGGGAACCCGGGAATTTTGATCATTTTGTTTCTTTTCGATCTGTTT
CTATTTTAAAAAGATGATGAAAGATCCGACTGGGTATAGATTTAGTCCGACGGGAGAGGA
AGTGATAAACCATTACCTAAAGAACAAAATTCTGGGTAAGACTTGGCTCGTTGATGAAGC
CATTAGCGAGATCAACATCTTGAATCACAAACCCAGCAAGGATTTGCCTAGTAATCTCTC
TCTCTCTCTCTGTGACTCTTTCTCTCTCGCTGTCTCGATCTATGTGTGTCTCTCTCTCTT
TCTCTCTCTCATTCTCTCTCTCACTCTCTTTCTCAC
   
Sequence Name 50-K033708-022-318-G08C-GC66
GenBank Accession LN507078 [GenBank]
Sequencing primer gene specific primer, GC66: 5'-GGGTGAGAAAGAGAGTGAGAGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K033708-022-318-G08C-GC66
ATCGAGACAGCGAGAGAGAAAGAGTCACAGAGAGAGAGAGAGAGATTACTAGGCAAATCC
TTGCTGGGTTTGTGATTCAAGATGTTGATCTCGCTAATGGCTTCATCAACGAGCCAAGTC
TTACCCAGAATTTTGTTCTTTAGGTAATGGTTTATCACTTCCTCTCCCGTCGGACTAAAT
CTATACCCAGTCGGATCTTTCATCATCTTTTTAAAATAGAAACAGATCGAAAAGAAACAA
AATGATCAAAATTCCCGGGTTCCCGGGATTAGGGTTTGAGTGAAGTTTTTTCGGCTTCTC
TCTCTCTGTGTTTTTCTTTCTTTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11