Sequences from confirmation amplicon


Confirmation sequences for line 320F09 and hit At1g73990 are shown.
Line ID 320F09
Locus At1g73990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27824442
Primers used for PCR KS45 - 8474, show primer details
   
Sequence Name 56-K033095-022-320-F09C-8409
GenBank Accession LN509017 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K033095-022-320-F09C-8409
CATTTACATTGAATATATCCTGCCGCCGCTGCCGCTTTGCACCCGGTGGAGCTTGCATGT
TGGTTTCTACGCAGAACTGAGCCGGTTAGGCAGATAATTTCCATTGAGAACTGAGCCATG
TGCACCTTCCCCCCAACACGGTGAGCGACGGGGCAACGGAGTGATCCACATGGGACTTTT
AAACATCATCCGCAGGTGAGAGAGAGAAACAATGGCGAAGCTACTTCTTCTTCACGCGCC
TCATGTAATACCTAGGTTTAGCAGCAGTAGTAGTAGATCACTGGTCTCCGCAGCGGCTTT
ATACAGAAGACCTCTCCTCGTGAATCCCCAATTTTCCCATATCGGACCTCGTCTTCATTC
TCCGTACAATCGGAGATTCTCGGCTCGAGCCTTCGACGATTCACCTGCTTCCTCGGCGGA
GATGGAGAAAGAGAAACAGGAACAGCTGCTGGATGGAGTTTCTGGGAAAAAAGATGAGGA
CTATCCGACCGGAGAAATGGAGTATGAGAATAGGAACGCGTGGGAGATTTTTGTT
   
Sequence Name 08-K033095-022-320-F09C-KS45
GenBank Accession LN489631 [GenBank]
Sequencing primer gene specific primer, KS45: 5'-ATCCGAAACTTCACAACAAAAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K033095-022-320-F09C-KS45
CTTTCTCCGGTCGGATAGTCCTCATCTTTTTTCCCAGAAACTCCATCCAGCAGCTGTTCC
TGTTTCTCTTTCTCCATCTCCGCCGAGGAAGCAGGTGAATCGTCGAAGGCTCGAGCCGAG
AATCTCCGATTGTACGGAGAATGAAGACGAGGTCCGATATGGGAAAATTGGGGATTCACG
AGGAGAGGTCTTCTGTATAAAGCCGCTGCGGAGACCAGTGATCTACTACTACTGCTGCTA
AACCTAGGTATTACATGAGGCGCGTGAAGAAGAAGTAGCTTCGCCATTGTTTCTCTCTCT
CACCTGCGGATGATGTTTAAAAGTCCCATGTGGATCACTCCGTTGCCCCGTCGCTCACCG
TGTTGGGGGGAAGGTGCACATGGCTCAGTTCTCAATGGAAATTATCTGCCTAACCGGCTC
AGTTCTGCGTAGAAACCAACATGCAAGCTCCACCGGGTGCAAAGCGGCAGCGGCGGCAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACNAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGT

Last Updated on 06.12.2018 16:11