Sequences from confirmation amplicon


Confirmation sequences for line 321C02 and hit At1g68590 are shown.
Line ID 321C02
Locus At1g68590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25758247
Primers used for PCR DE23 - 8474, show primer details
   
Sequence Name 01-K019548-022-321-C02C-DE23
GenBank Accession LN486385 [GenBank]
Sequencing primer gene specific primer, DE23: 5'-GAGATTGATGATATCAGTGGCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K019548-022-321-C02C-DE23
TCTTCTTCTGAGAGATCCATGGTTTAGCTTCAAGAGTAGACTTAAGCTCTTCCCAAGCAT
CTTTCCTAGGCCAGAAGAAGTAAGGACTAAGTGGGATTGTGGCATGGCCAGGGACATGGT
GGGCCAATCCTAATCCTATGGTCTTCTCCCATCCACACAAACTTGAGTACCACTCTTGAT
TTCTCATGTGGTTTCCCCCCCTAACACAAACAACAAATATTTGGGGGTTTTGTCACACGA
GACACAAGAATATGAAAAAAAATGTGGTGT
   
Sequence Name 02-K019548-022-321-C02C-8409
GenBank Accession LN486840 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K019548-022-321-C02C-8409
AGACATGAAGCCATTTACAATTGAATATATCCCCATTTTTCTCCACCCAAAAATGTGACC
ATATCTTCCTTATCCATATTCTCACAAAATCTCCATTGTTCTCTGAGAAAACACAAAAAT
GATGTCATCAATGGCACTCTCTTCTACTTCCCTCTGCTCTTCTTTCATCTCTCAACATCC
AAAATTCTCCATCACTGCTTCTCCTCCATTGTTCCATACCCAAACAACCAAACCCATCTC
TGTAAAAAGGAAAATCATAACTCTAGCAGCTCCAGAGACACTCACCGCTGAAACAGTCAC
CGGAATTGATACTTCCGACAACACTCCACAACAAACAATAAAGGTAAAAACAACATATTC
TTCTTCATCTTCTTGTCTCTTGTGTTACAAAACCCACAAATCTTTGTTGTTTGTGTTAGG
TGGTGAACCCAAATGAGAAATCAAGAGTGGTACTCAAGTTTGTGTGGATGGAGAACAACA
TAGGATTAGGATTGGACCAACATGTACCTGGACATGGCACAATCCCACTTAGTCCTTACT
TCTTCTGGCCTAAGA

Last Updated on 06.12.2018 16:11