Sequences from confirmation amplicon


Confirmation sequences for line 322B02 and hit At5g52470 are shown.
Line ID 322B02
Locus At5g52470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21295753
Primers used for PCR CK14 - 8474, show primer details
   
Sequence Name 37-K017887-022-322-B02C-CK14
GenBank Accession LN502440 [GenBank]
Sequencing primer gene specific primer, CK14: 5'-AGATGCTAGACATCCGGCTAAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K017887-022-322-B02C-CK14
ATGCCTCGTGGGCATGGTTGATGTCATATTCTCTGATGTTGCTCAGCCAGATCAGGTTTG
GTTCTATATTTCTTACTATGAAAGTTTGAAGTTACCCTTTCTACAGTTTTATTCACAAAG
GTTACGTCATATTGCATTGCATCTTTTGCTTTTAAAATATCAGTTCATTGAAATCGGCAT
ATGATGACATTTCATTTACTATGAAATGTTTGAAGCCTTTTTATTTTACAGTTATATCTT
CAAAGTTTTTTTTTATTGCATTGCATCTTTTGCTTTTAAAAAAATCAGTACAATGAAATC
GGCAGATGATGATTTTATGGGTAATTGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC
GTCCAAAT
   
Sequence Name 38-K017887-022-322-B02C-8409
GenBank Accession LN502817 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K017887-022-322-B02C-8409
TCCAATTACCCATAAAATCATCATCTGCCGATTTCATTGTACTGATTTTTTTAAAAGCAA
AAGATGCAATGCAATAAAAAAAAACTTTGAAGATATAACTGTAAAATAAAAAGGCTTCAA
ACATTTCATAGTAAATGAAATGTCATCATATGCCGATTTCAATGAACTGATATTTTAAAA
GCAAAAGATGCAATGCAATATGACGTAACCTTTGTGAATAAAACTGTAGAAAGGGTAACT
TCAAACTTTCATAGTAAGAAATATAGAACCAAACCTGATCTGGCTGAGCAACATCAGAGA
ATATGACATCAACCATGCCCACGAGCATTCTGTACTTAGCCGGATGTCTAGCATC

Last Updated on 06.12.2018 16:11