Sequences from confirmation amplicon


Confirmation sequences for line 323B07 and hit At5g67250 are shown.
Line ID 323B07
Locus At5g67250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26833577
Primers used for PCR KS02 - 8474, show primer details
   
Sequence Name 37-K023495-022-323-B07C-8409
GenBank Accession LN502467 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K023495-022-323-B07C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTTTTTTGACCGTGAA
GTAGTGTCTTGAGAACGTGAAGTTTGACCGTTGAACACGAGAAGTAATGATGGTCGCAGA
AGAAGAGCGTGACGAGAGGTTATAAATAAAGAGCAAAAAAACAAAAAAGTTAACAAAGTC
GAAGTAATCAACGATCACGATTAGCTCAACACTTATTTTAAGATCTGACGGTTGAAAAGA
AAGTGAATAAAGCGCGTGCGAGTCTGAGTTGGGTCGAGCGTGTCGACTACGATTATGACA
CGTGTTCCCTTATTTGACGGGATGAGCCGTTACTGTGTGTACTTTATAATTCCTCAAACC
AAAAAAACAACAAAAAATAAAATAAAGGGAAAAAAAAAAAATTGGAGAGGAAGAGAAAGG
AGGAAATCGAAACCTTTCGGTTGGTGATGGGCCAAGCGCCGTCTTCTACGGGGGAATCGA
ACGGACGAGAGTTAGATTTGAGGTTATGGTCTCCGGTGATTGTTGCTGGAGGTGAATCGA
TGGCTGTGGGGAATGTTGTGGATCGGGATTTCACCGGAGATCTGCCG
   
Sequence Name 38-K023495-022-323-B07C-KS02
GenBank Accession LN502843 [GenBank]
Sequencing primer gene specific primer, KS02: 5'-TCGAATCGATTGAACATAGAGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K023495-022-323-B07C-KS02
ATCTCATCCTTAGCATCTAGAGATAACCGATGACGACTCTGACCGTCGACGAGCAACCAC
CGTTTACAAACAAGAGAGCATCGCTTCCGATCTCCAGCTCCAAGAAACTGAAACACATGA
GCTAAACACTCATCCGGCAGATCTCCGGTGAAATCCCGATCCACAACATTCCCCACAGCC
ATCGATTCACCTCCAGCAACAATCACCGGAGACCATAACCTCAAATCTAACTCTCGTCCG
TTCGATTCCGCCGTAGACGACGGCGCTTGGCCCATCACCAACCGAATGGTTTCGATTTCC
TCCTTTCTCTTCCTCTCCAATTTTTTTTTTTTCCCTTTATTTAATTTTTGGTGGTTTTTT
GGGTTGGAGGAATTATAAAGTACACACGGTAACGGCTCATCCCGTCAAATAAGGGAACAC
GTGTCATAATCGTAGTCGACACGCTCGACCCAACTAAAACTCGCACGCGCTTTATTCACT
TTCTTTTCAACCGTCAGATCTTAAAATAAGTGTTGAGCTAATCGGGATCGTTGATTACTT
CGACTTTGTAAACTTTTTTGTTTTTTGGCCCTTAATTAATAACCTCTCGTCA

Last Updated on 06.12.2018 16:11