Sequences from confirmation amplicon


Confirmation sequences for line 323G04 and hit At3g54220 are shown.
Line ID 323G04
Locus At3g54220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20071969
Primers used for PCR AP01 - 8474, show primer details
   
Sequence Name 27-K022912-022-323-G04C-8409
GenBank Accession LN498201 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K022912-022-323-G04C-8409
AGATTTCCCGGACATGAAGCCAAGTTTCCAACTTTCTCAGCTAAAGGGCAGAACTCAAAA
GGCAGGCCAAGCTTTATCTGCCAAATCCGA
   
Sequence Name 31-K022912-022-323-G04C-AP01
GenBank Accession LN499985 [GenBank]
Sequencing primer gene specific primer, AP01: 5'-GAAGAGATTAAGAGGCAGAAGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K022912-022-323-G04C-AP01
AAAAGATTACACCTTCTCACATTGCTGCTACAGTGTGCTGAAGCTGTCTCTGCTGATAAT
CTCGAAGAAGCAAACAAGCTTCTTCTTGAGATCTCTCAGTTATCAACTCCTTACGGGACC
TCAGCGCAGAGAGTAGCTGCTTACTTCTCGGAAGCTATGTCAGCGAGATTACTCAACTCG
TGTCTCGGAATTTACGCGGCTTTGCCTTCACGGTGGATGCCTCAAACGCATAGCTTGAAA
ATGGTCTCTGCGTTTCAGGTCTTTAATGGGATAAGCCCTTTAGTGAAATTCTCACACTTT
ACAGCGAATCAGGCGATTCAAGAAGCATTTGAGAAAGAAGACAGTGTACACATCATTGAC
TTGGACATCATGCAGGGACTTCAATGGCCTGGTTTATTCCACATTCTTGCTTCTAGACCT
GGAGGACCTCCACACGTGCGACTCACGGGACTTGGTACTTCCATGGAAGCTCTTCAGGCT
ACAGGGAAACGTCTTTCGGATTTCGCAGATAAGCTTGGCCTGCCTTTTGAGTTCTGCCCT
TTAGCTGAGAAAGTTGGAAACTTGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAAT

Last Updated on 06.12.2018 16:11