Sequences from confirmation amplicon


Confirmation sequences for line 324D11 and hit At3g56400 are shown.
Line ID 324D11
Locus At3g56400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20909793
Primers used for PCR IS32 - 8474, show primer details
   
Sequence Name 54-K018157-022-324-D11C-IS32
GenBank Accession LN508329 [GenBank]
Sequencing primer gene specific primer, IS32: 5'-TAGGACTCTTGTGATCTTCGGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K018157-022-324-D11C-IS32
ATCTCATCATGATGGTCACAAGTCTTGCTCTTGGGAGTTTCTGCGTTGGTGTTACACGTG
TGGTTTCCTATGTATGTGATACTGAACATCTTGGGTTCGAGCTCAACCTTCTGGACTTGC
TTTGTTGCCTTGCACCCTTGGGTATACTTGTGTGTGCATCTAAAGTAACTTCTGCACAAC
ATAAATTAAAATAAAATAATTTAGGCCAAAATATATATGCCTCAAGCCCACAACCAATTA
AGCTCAGACCACATTTATGGTAGATATAATTGCGGTCCATGTGTAATAATACCCGGCAAC
ATGTCACATATCAAATACGAATATATATAGACAAAACTATATAGGGGTAAAAAGGACCTT
GGGAATTTGGCATTAAGAATCAATATGGAGAATGAGCAATGAAATCAATATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCA
GCGTTTTTATNAAATGAAAGTACATTTTGATAAAACGACNAATTACGATCCGTCGTATTT
ATTGGCGAAAGCAATAAACAAATTATT
   
Sequence Name 55-K018157-022-324-D11C-8409
GenBank Accession LN508657 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K018157-022-324-D11C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATATTGATTTCATTGCT
CATTCTCCATATTGATTCTTAATGCCAAATTCCCAAGGTCCTTTTTACCCCTATATAGTT
TTGTCTATATATATTCGTATCTGATATGTGACATGTTGCCGGGTATTATTACACATGGAC
CGCAATTATATCTACCATAAATGTGGTCTGAGCTTAATTGGTTGTGGGCTTGAGGCATAT
ATATTTTGGCCTAAATTATTTTATTTTAATTTATGTTGTGCAGAAGTTACTTTAGATGCA
CACACAAGTATACCCAAGGGTGCAAGGCAACAAAGCAAGTCCAGAAGGTTGAGCTCGAAC
CCAAGATGTTCAGTATCACATACATAGGAAACCACACGTGTAACACCAACGCAGAAACTC
CCAAGAGCAAGACTTGTGACCATCATGATGAGATCTTCATGGATTCCGAAGATCA
Primers used for PCR 89wr - 3144, show primer details
   
Sequence Name 89-K118835-0022-324-D11C-89wr
GenBank Accession LN514987 [GenBank]
Sequencing primer gene specific primer, 89wr: 5'-AAAAAGTGCCCATCATTACAAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K118835-0022-324-D11C-89wr
CACTTCTCTTCTTTCCTTTATCATACAAACCACACCAAGAGGAAAGAAAAATAAGATACC
ACTCACCAAAAACTTCCTCAAAACTAACAAATGGATACTAATAAAGCAAAAAAGCTTAAA
GTTATGAACCAACTCGTTGAAGGCCATGACTTAACAACTCAGCTTCAGCAACTCCTCTCT
CAACCCGGGTCCGGTCTAGAGGATCTAGTGGCTAAAATCTTAGTGTGTTTCAATAACACC
ATCTCCGTTCTTGATACCTTCGAACCCATCTCCTCCTCCTCATCCCTCGCCGCCGTTGAG
GGATCTCAAAATGCTTCATGTGATAACGACGGCAAGTTTGAAGATTCCGGCGATAGTCGG
AAAAGATTGGGACCCGTTAAGGGTAAAAGAGGATGCTACAAAAGAAAGTAAGTTTCGCCC
TTTTCTCTCTCTTACTCAATTATTAACCATAATGATATAAAATCAATCCATCGTAGTTCT
ATTAGACCGATTACGTTGATCATATGTACCATATATCTAGTGATCATCCATACTCATGGA
AAAAACTATCATCTAGGATTTACTTACTGTTATGGTTAGTCACAAACAAACAAGACATTT
AGCTTTGGTTGCAAGAATCATAAGTAGCCAAATGATTAATTGATTTCATTAATATTATGA
GCAGAAAGAGATCGGAGACGTGTACTATAGAGTCGACTATACTTGAGTAAAAGAACACTG
ATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAA
TGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCA
TCCCTTACG
   
Sequence Name 65-K118834-0022-324-D11C-35St
GenBank Accession LN511502 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K118834-0022-324-D11C-35St
ATCCACTATCTTGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAG
GGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAACTATCAGTGTTCTTTTAC
TCAAGTATAGTCGACTCTATAGTACACGTCTCCGATCTCTTTCTGCTCATAATATTAATG
AAATCAATTAATCATTTGGCTACTTATGATTCTTGCAACCAAAGCTAAATGTCTTGTTTG
TTTGTGACTAACCATAACAGTAAGTAAATCCTAGATGATAGTTTTTTCCATGAGTATGGA
TGATCACTAGATATATGGTACATATGATCAACGTAATCGGTCTAATAGAACTACGATGGA
TTGATTTTATATCATTATGGTTAATAATTGAGTAAGAGAGAGAAAAGGGCGAAACTTACT
TTCTTTTGTAGCATCCTCTTTTACCCTTAACGGGTCCCAATCTTTTCCGACTATCGCCGG
AATCTTCAAACTTGCCGTCGTTATCACATGAAGCATTTTGAGATCCCTCAACGGCGGCGA
GGGATGAGGAGGAGGAGATGGGTTCGAAGGTATCAAGAACGGAGATGGTGTTATTGAAAC
ACACTAAGATTTTAGCCACTAGATCCTCTAGACCGGACCCGGGTTGAGAGAGGAGTTGCT
GAAGCTGAGTTGTTAAGTCATGGCCTTCAACGAGTTGGTTCATAACTTTAAGCTTTTTTG
CTTTATTAGTATCCATTTGTTAGTTTTGAGGAAGTTTTTGGTGAGTGGTATCTTATTTTT
CTTTCCTCTTGGTGTGGTTTGTATGATTAAAGGAAAGAAGAGAAGTGTGTGTAAATATAA
GGTGGAAATAAGATTTGT

Last Updated on 06.12.2018 16:11