Sequences from confirmation amplicon


Confirmation sequences for line 325D02 and hit At2g02860 are shown.
Line ID 325D02
Locus At2g02860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:832269
Primers used for PCR MN01 - 8474, show primer details
   
Sequence Name 43-K020436-022-325-D02C-8409
GenBank Accession LN504689 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K020436-022-325-D02C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATAGCCGTATAGGAATTTGAGGA
AGGAAATTGAACTTGAGACGGTCACCAAGCATCGTCAAAACGAATCTGGTTCTTCGTCGT
TCTCTGAATCTGCTTCTCCTTCGAATCATTCTGATTCGGCTGATGGTGAATCTGTGTCGA
AGAATTGTAGTTTAGTGACGTTGGTTCTTAGTTGTACAGTTGCCGCTGGAGTTCAATTTG
GTTGGGCATTGCAACTTTCTCTTCTTACTCCTTATATTCAGGTACTTACTATTACTCTTG
TCATTGTTACCATTTCTATCACTTGTGGCTTTCTCTATAGCAATTTAGATTCGAGGGTTT
TGTTCTTGTGTAGCTTTGTACGAGAAAGTCGAGTACTTTGTAGGTGAATCAATGAATTCT
AATACTTAGGTTAGGTAGTTGTTGTCACCAACATAGCCATGAGATATTTTCGTATGGAAC
TCTATAGCATTGGCTTATCGAAGAGTATATGACATTGGAATTTAGCTAGATTGTTTATGT
TCATTGGATTACATAAGCATTTGTAAAAATATAAAAGACCTTAGCCTTTT
   
Sequence Name 44-K020436-022-325-D02C-MN01
GenBank Accession LN505039 [GenBank]
Sequencing primer gene specific primer, MN01: 5'-ATAGTTCCGAAAAAGGCTAAGGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K020436-022-325-D02C-MN01
TATTTTTACAAATGCTTATGTAATCCAATGAACATAAACAATCTAGCTAAATTCCAATGT
CATATACTCTTCGATAAGCCAATGCTATAGAGTTCCATACGAAAATATCTCATGGCTATG
TTGGTGACAACAACTACCTAACCTAAGTATTAGAATTCATTGATTCACCTACAAAGTACT
CGACTTTCTCGTACAAAGCTACACAAGAACAAAACCCTCGAATCTAAATTGCTATAGAGA
AAGCCACAAGTGATAGAAATGGTAACAATGACAAGAGTAATAGTAAGTACCTGAATATAA
GGAGTAAGAAGAGAAAGTTGCAATGCCCAACCAAATTGAACTCCAGCGGCAACTGTACAA
CTAAGAACCAACGTCACTAAACTACAATTCTTCGACACAGATTCACCATCAGCCGAATCA
GAATGATTCGAAGGAGAAGCAGATTCAGAGAACGACGAAGAACCAGATTCGTTTTGACGA
TGCTTGGTGACCGTCTCAAGTTCAATTTCCTTCCTCAAATTCCTATACGGCTATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGCAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTAAGGCGAAAGCAATAAACAAATTATTCTAATTCG

Last Updated on 06.12.2018 16:11