Sequences from confirmation amplicon


Confirmation sequences for line 325E04 and hit At2g41040 are shown.
Line ID 325E04
Locus At2g41040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17122605
Primers used for PCR CK46 - 8474, show primer details
   
Sequence Name 56-K018157-022-325-E04C-CK46
GenBank Accession LN508967 [GenBank]
Sequencing primer gene specific primer, CK46: 5'-CTTGATCTCATCCTGCATCAAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K018157-022-325-E04C-CK46
CAGAATTCTCTGCAAAAATGCAAAATGTATAAAGATCAAAATCTATGCATAGAAATAGCC
ACTTTTGATCATTATAAATTTTTCTTTTACCGAATTGGAAA
   
Sequence Name 57-K018157-022-325-E04C-8409
GenBank Accession LN509269 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K018157-022-325-E04C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTAACGTTAATATAGC
TGTAATATACATGGGTTCTGTTGACGCGGTTCATGCTGGTGCAGCCTTGCACTGTTGGCC
TTCTCCTACAAATGCTGTAAGTTCTTGAATATGTCAGAGCTTCATGAGTGTATGCAAATG
GTCACTTGTTAAATTTGTCTTTGTGTTGTTGTTTTCAAGATTGCTGAGATCTGTCGTGTT
CTAAGAAGCGGAGGTGTGTTTGTCGGGACAACGTTTCTCAGATACAGTCCTTCGACTCCT
TGGATTATTCGTCCTTTCCAATCGGTAAAGAAAATTTATAATGATCAAAAGTGGCTATTT
CTATGCATAGATTTTGATCTTTATACATTTTGCATTTTTGCAGAGAATTCTGCAGAGCTA
CAATTACTTGATGCA

Last Updated on 06.12.2018 16:11