Sequences from confirmation amplicon


Confirmation sequences for line 326A05 and hit T20K14 are shown.
Line ID 326A05
Locus T20K14 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5027587
Primers used for PCR MP36 - 8474, show primer details
   
Sequence Name 09-K023017-022-326-A05C-8409
GenBank Accession LN489995 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K023017-022-326-A05C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTTAACAGAACATCTTTTACTCTTTTTAGT
AACTGCAGTAGAAATCAACTTGGGAAAACTTTTATATTATGAAAGTAATATTCGAAAAGC
TTACAATATTTGATAACCCAAGGTACATAATTACCAACAAGAACCAAACCTAAAATCTCT
GTAGGAATTCATAATAGGAAGAACAAAAGAAACAATTATTGATCAAACATACTGTAGTTA
AAGATATATGGAAAAACTCATGAGAATTCGGAAAAAATGGAGAAAAAAGCTAAGGAAGAG
GTTTGGTTGTTGTTTAGGCAAGAGCAGGCATGTCCTTGAGCCACTGGTCCAATGGCTTAC
CAATGGAGTAAACAATAAACCCAATCTCCCTGAGTTTATCAGCGTCGACCACGTTTCTAC
CGTCAAAAACAAAAGCCGGTTTCTGCATATTCTCAAAGATCCGCTGGAAATCAAGTTTCT
TGAACTCGTCCCACTCGGTTAAAATGCAGATACCGTGGGCGTCTTTGGTTGCAGTGTATG
CGTCCCAAGCGACTGAGACTTGCTTCACAGTGGTGGGGCTCATGGGCTGGAGATGAAGTG
GGTGGTCCCAGTCGAATTTGTTCATGGTTAA
   
Sequence Name 10-K023017-022-326-A05C-MP36
GenBank Accession LN490468 [GenBank]
Sequencing primer gene specific primer, MP36: 5'-GCAGATCCAAAGAGACTTAACCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K023017-022-326-A05C-MP36
AATTCGACTGGGACCACCCACTTCATCTCCAGCCCATGAGCCCCACCACTGTGAAGCAAG
TCTCAGTCGCTTGGGACGCATACACTGCAACCAAAGACGCCCACGGTATCTGCATTTTAA
CCGAGTGGGACGAGTTCAAGAAACTTGATTTCCAGCGGATCTTTGAGAATATGCAGAAAC
CGGCTTTTGTTTTTGACGGTAGAAACGTGGTCGACGCTGATAAACTCAGGGAGATTGGGT
TTATTGTTTACTCCATTGGTAAGCCATTGGACCAGTGGCTCAAGGACATGCCTGCTCTTG
CCTAAACAACAACCAAACCTCTTCCTTAGCTTTTTTCTCCATTTTTTCCGAATTCTCATG
AGTTTTTCCATATATCTTTAACTACAGTATGTTTGATCAATAATTGTTTCTTTTGTTCTT
CCTATTATGAATTCCTACAGAGATTTTAGGTTTGGTTCTTGTTGGTAATTATGTACCTTG
GGTTATCAAATATTGTAAGCTTTTCGAATATTACTTTCATAATATAAAAGTTTTCCCAAG
TTGATTTCTACTGCAGTTACTAAAAAGAGTAAAAGATGTTCTGTTAAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
AAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAA
GCAATAAACAAATTATTCTAATTC

Last Updated on 06.12.2018 16:11