Sequences from confirmation amplicon


Confirmation sequences for line 326D01 and hit At4g08310 are shown.
Line ID 326D01
Locus At4g08310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5252183
Primers used for PCR PA76 - 8474, show primer details
   
Sequence Name 47-K037018-022-326-D01C-8409
GenBank Accession LN506126 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K037018-022-326-D01C-8409
ACTCTTTCATTTACACTCGTCAGTGGGATGGGCATTCGGGTAAGCGGGTCGGGTTCGGTT
AAAACCCGATCGGTTACAGATTTTTCGGGTAGAAAAGTTTAGAACCCAGGTGGATAAATC
TAAAATATCGGTTCGATTTCGGTTCGCATCTAATCGGGTTCGGATTAGTTTGGGTATAGA
TTTTTGGGCCCTATTTTGGAACTTCGGGTAAATATCCGGATTTTTGAGTAAAATTTCAGG
TTTTAGGTAAAATTTAGATTTTCAATTAAAATATTCGGGTATTTTTAGTTTTGGGTTCGG
GTTTGATTTTTCGGATAGAAGAATTTAAGACCTAATAGAATAAATCTTCGAATTCAGATT
CGGTTTTAGATCCAACTTTTCGGGCCAATTTCGATTCGGGTTGTTGGTTTCGGGTTTTTT
TCCCAGCCCTACTCATCACTTCTTCAGTCTGGGACGCGAGGACGTCCAACAGGCTAAAAT
TAGCGGAACCAGATGAGGACAAGGACGTAAAATTATATAGTTTATTTGTTTGTTAATCAG
CTTAATATAACTCTATACCCAACCAGAACTATAATAACCTTGTATCCAGACAGACTTTTC
AATTGCCTCCTTCAACGAACACTTTCATCGCAGAGAAGAAGAAACCAGATTCTTCTCCTT
CTCTCTTTCTCTCTTCCTCCAATGGCGAA
   
Sequence Name 48-K037018-022-326-D01C-PA76
GenBank Accession LN506435 [GenBank]
Sequencing primer gene specific primer, PA76: 5'-AGAGTCTGTTGTTAGTTTCGCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K037018-022-326-D01C-PA76
ATCTGGTTTCTTCTTCTCTGCGATGAAAGTGTTCGTTGAAGGAGGCAATTGAAAAGTCTG
TCTGGATACAAGGTTATTATAGTTCTGGTTGGGTATAGAGTTATATTAAGCTGATTAACA
AACAAATAAACTATATAATTTTACGTCCTTGTCCTCATCTGGTTCCGCTAATTTTAGCCT
GTTGGACGTCCTCGCGTCCCAGACTGAAGAAGTGATGAGTAGGGCTGGGAAAAAAACCCG
AAACCAACAACCCGAATCGAAATTGGCCCGAAAAGTTGGATCTAAAACCGAATCTGAATT
CGAAGATTTATTCTATTAGGTCTTAAATTCTTCTATCCGAAAAATCAAACCCGAACCCAA
AACTAAAAATACCCGAATATTTTAATTGAAAATCTAAATTTTACCTAAAACCTGAAATTT
TACTCAAAAATCCGGATATTTACCCGAAGTTCCAAAATAGGGCCCAAAAATCTATACCCA
AACTAATCCGAACCCGATTAGATGCGAACCGAAATCGAACCGATATTTTAGATTTATCCA
CCTGGGTTCTAAACTTTTCTACCCGAAAAATCTGTAACCGATCGGGTTTTAACCGAACCC
GACCCGCTTACCCGAATGCCCATCCCACTGACGAGTGTAAATGAAAGAGTATGATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATT

Last Updated on 06.12.2018 16:11