Sequences from confirmation amplicon


Confirmation sequences for line 326G12 and hit At3g50890 are shown.
Line ID 326G12
Locus At3g50890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18917100
Primers used for PCR NT14 - 8474, show primer details
   
Sequence Name 61-K036524-022-326-G12C-NT14
GenBank Accession LN510450 [GenBank]
Sequencing primer gene specific primer, NT14: 5'-AACCAACTGAGCTATTCCAGCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K036524-022-326-G12C-NT14
GCATTAATTATTAATTACACACGTTTTTCCCTCTTTATTTGAAGGTCCAATTTTATCACT
TTGTTCAACCACAAATTAAGTAAAAGAAAAAAATAATCCCAGATACAATATAAAACAATC
AAAACATACAAGATTCCACAGATTTATACTCAAGATTTTGAATAATTTGAAGGGCAAAAA
TTATATATAAAAGCAAAACAGGATTGTAAAAATTTATTTCACTTTTATCAGATCATCAAA
CTATTCACTAATGTTACTATTGTTTCTCTTCATTGCTTGCTTGTTATTATGCATCCAAAC
CTTAAAAACTTGTAAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTCAC
   
Sequence Name 37-K036524-022-326-G12C-8409
GenBank Accession LN502531 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K036524-022-326-G12C-8409
GCCATTTACATTTACAGTTTTTAAGGTTTGGATGCATAATAACAAGCAAGCAATGAAGAG
AAACAATAGTAACATTAGTGAATAGTTTGATGATCTGATAAAAGTGAAATAAATTTTTAC
AATCCTGTTTTGCTTTTATATATAATTTTTGCCCTTCAAATTATTCAAAATCTTGAGTAT
AAATCTGTGGAATCTTGTATGTTTTGATTGTTTTATATTGTATCTGGGATTATTTTTTTC
TTTTACTTAATTTGTGGTTGAACAAAGTGATAAAATTGGACCTTCAAATAAAGAGGGAAA
AACGTGTGTAATTAATAATTTAATGCACAAGTATGGTTAGTACAATTAGCTGGAATAGCT
CAGTTGG

Last Updated on 06.12.2018 16:11