Sequences from confirmation amplicon


Confirmation sequences for line 327D04 and hit At3g26450 are shown.
Line ID 327D04
Locus At3g26450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9681940
Primers used for PCR PW13 - 8474, show primer details
   
Sequence Name 31-K024603-022-327-D04C-8409
GenBank Accession LN499998 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K024603-022-327-D04C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATAAATATGACAATAATTTAA
TTTACACGCTTTAACAAAAAAAAAGAAAAAAATAATGATTTACATGTATGTAAAGATGGA
AAGGAGGAGGTATTCAAGGAGAGGAGAGAGATAGACGATGACAATAAGATAGTGAAGGTT
GTAGGATTGGAAGGTCACGTGATGGAGCAGTTCAAGGTGTATGAGATTGACTTCCAATTT
ATTCCCAACTCTGAAGAAGATTGCGTCTGCAAAATCACTATGATATGGGAGAAGCGCAAC
GATGATTTCCCCGAACCAAGCAGCTACATGCAACTCCTCAAGAGTATGGTTATTGATATG
GAGGACCACGTCCTTAAAGCTTAATCAATATCACAACCACCACCATCATCACAACCACCA
TCACCATCATCCTATATATTTATTAAATTGTTTTCATTTATATATAATAGACTAGATAAG
AACCTGTA
   
Sequence Name 32-K024603-022-327-D04C-PW13
GenBank Accession LN500422 [GenBank]
Sequencing primer gene specific primer, PW13: 5'-CCCGCACATTATACAGGTTCTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K024603-022-327-D04C-PW13
CTAGTCTATTATATATAAATGAAAACAATTTAATAAATATATAGGATGATGGTGATGGTG
GTTGTGATGATGGTGGTGGTTGTGATATTGATTAAGCTTTAAGGACGTGGTCCTCCATAT
CAATAACCATACTCTTGAGGAGTTGCATGTAGCTGCTTGGTTCGGGGAAATCATCGTTGC
GCTTCTCCCATATCATAGTGATTTTGCAGACGCAATCTTCTTCAGAGTTGGGAATAAATT
GGAAGTCAATCTCATACACCTTGAACTGCTCCATCACGTGACCTTCCAATCCTACAACCT
TCACTATCTTATTGTCATCGTCTATCTCTCTCCTCTCCTTGAATACCTCCTCCTTTCCAT
CTTTACATACATGTAAATCATTATTTTTTTCTTTTTTTTTGTTAAAGCGTGTAAATTAAA
TTATTGTCATATTTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11