Sequences from confirmation amplicon


Confirmation sequences for line 330E08 and hit At4g38520 are shown.
Line ID 330E08
Locus At4g38520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18016412
Primers used for PCR DL67 - 8474, show primer details
   
Sequence Name 20-K018513-022-330-E08C-DL67
GenBank Accession LN495012 [GenBank]
Sequencing primer gene specific primer, DL67: 5'-GCAACAGTGTATGTCATCAGAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K018513-022-330-E08C-DL67
AAAAAAGCGTTCCAAGCCACTGAAGAAGGCTTCTTATCCATAGTTACAAATCAATTTCAA
ACTAGACCTCAGATAGCCACAGTGGGATCATGCTGTCTTGTAAGTGTCATCTGCGATGGG
AAGCTATACGTGGCCAACGCAGGGGACTCACGGGCCGTTCTGGGACAAGTCATGAGGGTA
ACAGGTGAAGCTCATGCCACTCAGCTCTCAGCAGAGCACAACGCATCTATAGAGTCAGTG
AGACGGGAACTTCAGGCCCTGCATCCGGATCATCCAGATATTGTGGTTCTGAAACATAAC
GTCTGGCGAGTAAAAGGCATCATTCAGGTATTTAATAAACTAGAGTTGGACTAAATCTGT
TTTTGTATGTCTTACAATTCCTGTTGTCTTTCTGAGTGGCTTTATGACTATACTCAGGTT
TCAAGATCCATTGGTGATGTGTATTTGAAAAGGTCAGAGTTCAACAGGGAACCACTGTAT
GCAAAATTCCGGCTGAGGTCACCGTTCAGCAAGCCATTACTGAGTGCAGAGCCGGCGATC
ACGGTGCATACACTGGAGCCGCACGATCAGTTCATTATATGTGCATCAGATGGACTGTGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATT
   
Sequence Name 21-K018513-022-330-E08C-8409
GenBank Accession LN495463 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K018513-022-330-E08C-8409
ATTTCCCACAGTCCATCTGATGCACATATAATGAACTGATCGTGCGGCTCCAGTGTATGC
ACCGTGATCGCCGGCTCTGCACTCAGTAATGGCTTGCTGAACGGTGACCTCAGCCGGAAT
TTTGCATACAGTGGTTCCCTGTTGAACTCTGACCTTTTCAAATACACATCACCAATGGAT
CTTGAAACCTGAGTATAGTCATAAAGCCACTCAGAAAGACAACAGGAATTGTAAGACATA
CAAAAACAGATTTAGTCCAACTCTAGTTTATTAAATACCTGAATGATGCCTTTTACTCGC
CAGACGTTATGTTTCAGAACCACAATATCTGGATGATCCGGATGCAGGGCCTGAAGTTCC
CGTCTCACTGACTCTATAGATGCGTTGTGCTCTGCTGAGAGCTGAGTGGCATGAGCTTCA
CCTGTTACCCTCATGACTTGTCCCAGAACGGCCCGTGAGTCCCCTGCGTTGGCCACGTAT
AGCTTCCCATCGCAGATGACACTTACAAGACAGCATGATCCCACTGTGGCTATCTGAGGT
CTAGTTTGAAATTGATTTGTAACTATGGATAAGAAGCCTTCTTCAGTGGCTTGGAACGCT
TTTTTTATCACCTCTGATGA

Last Updated on 06.12.2018 16:11