Sequences from confirmation amplicon


Confirmation sequences for line 331D12 and hit At5g62040 are shown.
Line ID 331D12
Locus At5g62040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24923355
Primers used for PCR AC10 - 8474, show primer details
   
Sequence Name 21-K027789-022-331-D12C-8409
GenBank Accession LN495524 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K027789-022-331-D12C-8409
AGCCATTTACATTGAATATATCCTGCATATATCCTCGTCGAGCGCGTCTTACATGCATAT
ATCATGCAATGATCGTCGAACATGCATATATCTTTGGTGGTGGGTGAAAAAGTCTATGTA
AATGTGTTATGATGTGATGTATTCTTACCAAAAGAAGCATCGGTTGTCCCGGGAATATCT
GTCACCATCCTGAATTTGATGAGAACATAAAGAATACATATTATACATTGTTGTTGTAAT
ATATGAGAAGTAATGTGATATCTTCAATCGACTTACCAATGCAGATATTCACGCATATAA
GGATTACTAGGACTCGGGGCATCGGGGTCCATCATGATCTTTCGAGTTAAAAAGAAAACC
ACAAGATTAATGCTACAAGAGAGAACTAAATAAGGTACTTAACATATGTATTTTAAGTTT
AGAAGAAAGCTAATTACGTACTAAGGTGAAGAAGGAACGAAGATCTTGGCCACCGATCTC
AACGCGAGGCTTAGAGAGGAGAAGAGAAGGCGCGAGCTCGTGACCATTGGATACGATTGT
GTTGGAATTGAAAGTGACTCTCATTGTCACACTTGGATTAAACATTTCGAGTACATCTCC
TATCACTCTTCCCACTATTAGTGGCTCTATTTCTCTTGACATTTTTCTTTGCTCCAATGT
GTTTGCGTTTGTGA
   
Sequence Name 22-K027789-022-331-D12C-AC10
GenBank Accession LN495970 [GenBank]
Sequencing primer gene specific primer, AC10: 5'-TCTAACGAAATCAGCATCAATCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K027789-022-331-D12C-AC10
TGTCAAGAGAAATAGAGCCACTAATAGTGGGAAGAGTGATAGGAGATGTACTCGAAATGT
TTAATCCAAGTGTGACAATGAGAGTCACTTTCAATTCCAACACAATCGTATCCAATGGTC
ACGAGCTCGCGCCTTCTCTTCTCCTCTCTAAGCCTCGCGTTGAGATCGGTGGCCAAGATC
TTCGTTCCTTCTTCACCTTAGTACGTAATTAGCTTTCTTCTAAACTTAAAATACATATGT
TAAGTACCTTATTTAGTTCTCTCTTGTAGCATTAATCTTGTGGTTTTCTTTTTAACTCGA
AAGATCATGATGGACCCCGATGCCCCGAGTCCTAGTAATCCTTATATGCGTGAATATCTG
CATTGGTAAGTCGATTGAAGATATCACATTACTTCTCATATATTACAACAACAATGTATA
ATATGTATTCTTTATGTTCTCATCAAATTCAGGATGGTGACAGATATTCCCGGGACAACC
GATGCTTCTTTTGGTAAGAATACATCACATCATAACACATTTACATAGACTTTTTCACCC
ACCACCAAAGATATATGCATGTTCGACGATCATTGCATGATATATGCATGTAAGACGCGC
TCGACGAGGATATATGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATT

Last Updated on 06.12.2018 16:11