Sequences from confirmation amplicon


Confirmation sequences for line 332A10 and hit At1g22460 are shown.
Line ID 332A10
Locus At1g22460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7930210
Primers used for PCR NT17 - 8474, show primer details
   
Sequence Name 80-K036525-022-332-A10C-8409
GenBank Accession LN513907 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K036525-022-332-A10C-8409
GAGCCATTTACATTGAATATATCCTGTAATTGATTAACCATAACCATCTCTGTTTTCTTG
ATCTGTGTTAGTGTTCTTATTCAGTTTCTGAGACTGGGTCGTTTCTTGATTTGATTCTAT
GTTTAACAGCGAGGATATATCATAGATTACAATAAATCCATCACAGAACAGAGTTTAACG
AGGAATCTGTCTCGTCCGGAATCTAATGAAATTCCTCTCATCTCCAATCCCAAGGTTCAA
TGTTTACAATGTTTTTCGAGATCTTCTTGTACTGAGACAAGTTTTGCACTTTAGCTATCA
ATATTGTTTCTTTTTTAACTTGTGACTGATTCTTTGTCAAATTTCAGACAAATGAGATTC
AGTATCAAAGTTCGATCTCGGAACACATCAACAACACCGAGCTAGTTCCTCCTCATGTGT
CTACAAGCCCTTCTTCTTCTTCCAGGGTTT
   
Sequence Name 78-K036525-022-332-A10C-NT17
GenBank Accession LN513639 [GenBank]
Sequencing primer gene specific primer, NT17: 5'-CTCAAACCTTGGAAGAAGAAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K036525-022-332-A10C-NT17
GCTCGGTGTTGTTGATGTGTTCCGAGATCGAACTTTGATACTGAATCTCATTTGTCTGAA
ATTTGACAAAGAATCAGTCACAAGTTAAAAAAGAAACAATATTGATAGCTAAAGTGCAAA
ACTTGTCTCAGTACAAGAAGATCTCGAAAAACATTGTAAACATTGAACCTTGGGATTGGA
GATGAGAGGAATTTCATTAGATTCCGGACGAGACAGATTCCTCGTTAAACTCTGTTCTGT
GATGGATTTATTGTAATCTATGATATATCCTCGCTGTTAAACATAGAATCAAATCAAGAA
ACGACCCAGTCTCAGAAACTGAATAAGAACACTAACACAGATCAAGAAAACAGAGATGGT
TATGGTTAATCAATTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATTTCGACGTGTCTACATTCACT
Primers used for PCR NT16 - 8474, show primer details
   
Sequence Name 92-K036621-022-332-A10C-NT16
GenBank Accession LN515167 [GenBank]
Sequencing primer gene specific primer, NT16: 5'-CGTGACCCTTCTCTAACCTTTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K036621-022-332-A10C-NT16
GAACAGCGAAAGACAAACTAAACTTTGGAAAAAGATCGTCGATTTTTGGGCATTCATCAT
CGTGGCATTGATTGATTGATTGGCATCTCATACTTTCCTTTTTCGGCTAATCTCTACTCC
CAAAAATAGAAAGTCTCATTCACTCACCAAGCGAAAACAGAACAAGAAGAAGATTCAAGA
TCCGTTTTTTCTGATTCTTTTCTTTGACGAGCGGTTTGCTTTTCCTGAATGTTTCCGGTT
GGATCTTTCACATAAGAAATGCGGCGGCGGAGGAAGACGGTTGTGGTGGCGGTAGTGATA
CGGAGGGTGCTAATATGGGCCATATGCGTAATGACATTACTCTGTTTTCTCACCGTACAC
ATCTATGTTGCTCCTTTCAATAGACTCCCTAAGCTTCACCGGGTGCAAAGCGGCAGCGGC
GGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACATTCA
   
Sequence Name 91-K036621-022-332-A10C-8409
GenBank Accession LN515091 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K036621-022-332-A10C-8409
CATTTACATTGAATAATCCTGCCGCCGCTGCCGCTTTGCACCCGGTGAAGCTTAGGGAGT
CTATTGAAAGGAGCAACATAGATGTGTACGGTGAGAAAACAGAGTAATGTCATTACGCAT
ATGGCCCATATTAGCACCCTCCGTATCACTACCGCCACCACAACCGTCTTCCTCCGCCGC
CGCATTTCTTATGTGAAAGATCCAACCGGAAACATTCAGGAAAAGCAAACCGCTCGTCAA
AGAAAAGAATCAGAAAAAACGGATCTTGAATCTTCTTCTTGTTCTGTTTTCGCTTGGTGA
GTGAATGAGACTTTCTATTTTTGGGAGTAGAGATTAGCCGAAAAAGGAAAGTATGAGATG
CCAATCAATCAATCAATGCCACGATGATGAATGCCCAAAAATCGACGATCTTTTTCCAAA
GTTTAGTTTGGTCTTTTCGCTTGTTTTCTATTTTAAGAGTTAAGAAGCTAAAAAGGTTAG
AAAGGGGTCACG

Last Updated on 06.12.2018 16:11