Sequences from confirmation amplicon


Confirmation sequences for line 334D05 and hit At5g13150 are shown.
Line ID 334D05
Locus At5g13150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4173900
Primers used for PCR 12mv - 8474, show primer details
   
Sequence Name 11-K114418-0022-334-D05C-8409
GenBank Accession LN491216 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K114418-0022-334-D05C-8409
GCATTTCATTGAATTGTATATTCTGTACATATTGTTTACATGTACGAGACGCTTCGTGAT
TTGATTCCTGCGGTGGAACAATCTGATTCAGATTTGATCCAGGAGATTAAACTGGCTCAG
ACGCGGCTAGGAGAAGCAGCAGTGACGATATTCGGAGAGCTAGAGAAGTCGATAAAGAGC
GATAATGGACGAACTCCGGTACCGAGCGGCGCAGTTCATCCATTAACACGTTACACCATG
AACTACCTCAAATACGCTTGTGAGTATAAAGAAACATTAGACCAAGTGTTCCAACACTAT
GAAGCGAATCAAACAGATNACAAACCTGAACCAGAGACCAAACCTAGGCAGCAGCAACGA
GAAGACGATGAAGAGTACAAAGTTCAGGCTTTCG
   
Sequence Name 35-K114419-0022-334-D05C-12mv
GenBank Accession LN501928 [GenBank]
Sequencing primer gene specific primer, 12mv: 5'-GCGAAAGCTGAAACTTTGTACTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K114419-0022-334-D05C-12mv
GGTCTCTGGTTCAGGTTTGTTATCTGTTTGATTCGCTTCATAGTGTTGGAACACTTGGTC
TAATGTTTCTTTATACTCACAAGCGTATTTGAGGTAGTTCATGGTGTAACGTGTTAATGG
ATGAACTGCGCCGCTCGGTACCGGAGTTCGTCCATTATCGCTCTTTATCGACTTCTCTAG
CTCTCCGAATATCGTCACTGCTGCTTCTCCTAGCCGCGTCTGAGCCAGTTTAATCTCCTG
GATCAAATCTGAATCAGATTGTTCCACCGCAGGAATCAAATCACGAAGCGTCTCGTACAT
GTAAACAATATGTACATGAATATACATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAATTATA

Last Updated on 06.12.2018 16:11